TEST ORDERING INSTRUCTIONS:
Fill out test request form fully & legibly. Provide your contact information & complete history including suspected diagnosis & relevant investigation details (lab, imaging). Specify the exact sample type, procedure done, anatomic site, laterality where applicable (e.g. left vs right) and the fixative used.
SAMPLE TYPE:
A variety of specimens are categorized as ""large"" based on hybrid criteria (size/ complexity/ cost of processing/ pathologist time) e.g. whole organ(s), large amputation, or a large biopsy of part of an organ. Examples: prostatectomy, mastectomy, colectomy, esophagectomy, gastrectomy, extremity (limb) amputation or disarticulation, nephrectomy, laryngectomy, hepatectomy, lung resection, splenectomy, hysterectomy (with or without adnexae), lung (segment, lobar or total) resection, pancreas, vulvectomy specimens. Also, soft tissue lesions ≥5 cm in maximum dimension, bone resections for neoplasms, eye enucleation or exenteration, gall bladder & porta hepatis radical resection etc.
CONTAINER/ PRESERVATIVE
10% formalin (10% neutral buffered formalin preferred) in appropriate size, leak proof container. Immerse specimen into formalin within 30 minutes of removal. Ensure specimen is immersed in fixative & not stuck on the side/ lid of the container. For guidance on how to prepare 10% neutral buffered formalin, see this video: Youtube Video
SAMPLE VOLUME:
Volume of formalin to tissue of ≥10:1
Clinical Utility
Histopathologic examination of tissue involves microscopic analysis of biopsy or surgical specimens. It's crucial for:
1. Diagnosing diseases e.g. cancers.
2. Determining disease stage and grade.
3. Assessing surgical margins in cancer removal.
4. Identifying infections/ infectious agents.
5. Evaluating treatment efficacy.
6. Guiding treatment decisions.
7. Providing prognostic information (predicting how a disease might progress).
8. Supporting clinical-pathological correlation.
9. Aiding in research and understanding disease mechanisms.
This test offers vital insights for patient care and treatment planning.