Category

SEROLOGY/ IMMUNOLOGY

Sub Category

INFECTIOUS

Synonyms/Aliases

SALMONELLA TYPHI - O & H ANTIGEN, SALMONELLA PARATYPHI A - H ANTIGEN, SALMONELLA PARATYPHI B - H ANTIGEN.

Abbreviations

N/A

Type

PROFILE/ PANEL (BUNDLED TESTS).

4-5 Days

4-5 WORKING DAYS

Method

AGGLUTINATION.

Temp

AMBIENT (24 HOURS), REFRIGERATED (7 DAYS), FROZEN (30 DAYS).

Setup

ALL WORKING DAYS.

Components Parameters

"O" ANTIGEN, "H" ANTIGEN.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM.

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

2 ML

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.

Other Instructions

CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.

Reference intervals

NEGATIVE.

Clinical Utility

DETECTS THE PRESENCE OF SERUM AGGLUTININS (H AND O) IN PATIENTS SERUM WITH TYPHOID AND PARATYPHOID FEVER.

Test Limitations/ Confounders

CROSS-REACTIVITY WITH OTHER SALMONELLA SPECIES. INABILITY TO DISTINGUISH CURRENT VS. PREVIOUS INFECTION VS. VACCINATION AGAINST TYPHOID.