Haematology – Page 2 – ScanLab Center

10 May

HUMAN LEUCOCYTE ANTIGEN B27

May 10, 2023May 11, 2023vincentochiengde

Category

Hematology

Sub Category

Flow Cytometry

Synonyms/Aliases

N/A

Abbreviations

HLA B27

^Type

Individual test

^{7 days}

Results ready in 7 days

^Method

Flow cytometry.

^Temp

Room temperature.

^Setup

Week Days

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA whole blood.

Container/ preservative

EDTA (Purple Top) Vacutainer.

Sample volume

3ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted sample.

Other Instructions

N/A

Reference intervals

Age appropriate values provided with report.

Clinical Utility

Ankylosing Spondylitis, Juvenile Rheumatoid Arthritis and Reiter Syndrome.

Test Limitations/ Confounders

N/A

10 May

HAEMOGLOBIN

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

General.

Synonyms/Aliases

Hemoglobin

Abbreviations

^Type

Individual Test

^{3 Hrs}

Results ready Within 3 Hours.

^Method

Cyanmethemoglobin or SLS Hemoglobin detection method.

^Temp

Room Temperature (Avoid regrigeration)

^Setup

Daily

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA whole blood.

Container/ preservative

EDTA (PURPLE TOP) Vacutainers.

Sample volume

3ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted samples.

Other Instructions

N/A

Reference intervals

Age & gender appropriate values provided with report.

Clinical Utility

Screening test, reveals if there is any abnormal or immature cells.

Test Limitations/ Confounders

Lipemia, presence of Cryoglobulins can result In spurious HB Results.

10 May

FLOW CYTOMETRY, LYMPHOCYTE SUBSET PANEL

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Flow Cytometry

Synonyms/Aliases

N/A

Abbreviations

Flow Cytometry Immunophenotyping.

^Type

Profile/ Panel (Bundled Tests).

^{5 Days}

Results ready Within 5 days.

^Method

Flow Cytometry Immunophenotyping

^Temp

Room Temperature (Avoid regrigeration)

^Setup

Weekdays

Components Parameters

Variable markers e.g. CD3, 4, 8 (T CELL MARKER), CD19 (B CELL MARKERS) etc.

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly. Clinical history must be provided.

Patient preparation

N/A

Sample type

EDTA whole blood.

Container/ preservative

EDTA (PURPLE TOP) Vacutainers.

Sample volume

3ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted samples.

Other Instructions

N/A

Reference intervals

Age & gender appropriate values are provided on the report.

Clinical Utility

Evaluation of suspected congenital or acquired immunodeficiency.

Test Limitations/ Confounders

Samples are time sensitive & should be promptly delivered to the lab.

10 May

FLOW CYTOMETRY, CHRONIC LEUKEMIA/ LYMPHOMA PANEL

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Flow Cytometry

Synonyms/Aliases

N/A

Abbreviations

N/A

^Type

Profile/ Panel (Bundled Tests).

^{5 Days}

Results ready Within 5 days.

^Method

Flow Cytometry Immunophenotyping

^Temp

Room Temperature (Avoid regrigeration)

^Setup

MON-FRI

Components Parameters

Variable markers to assess clonality & lineage e.g. KAPPA, LAMBDA (MARKERS OF CLONALITY), CD3 (T CELL MARKER), CD19/ CD20 (B CELL MARKERS) etc.

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly. Clinical history, CBC, PBF report findings must be provided. Sample type must be specified, I.E. Peripheral blood vs. Bone marrow. The specific panel required (Acute Leukemia vs. Chronic Leukemia/ Lymphoma) must be specified based on outcome of baseline investigations.

Patient preparation

N/A

Sample type

EDTA whole blood/ bone marrow plus Heparin whole blood/ bone marrow plus well prepared, labeled & fixed slides.

Container/ preservative

EDTA (PURPLE TOP) & HEPARIN (GREEN TOP) vacutainers. slides should be prepared from the EDTA vacutainer.

Sample volume

3ML Each

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted samples.

Other Instructions

N/A

Reference intervals

Negative.

Clinical Utility

Classification and diagnosis of Chronic Leukemia/ Lymphoma.

Test Limitations/ Confounders

Samples are time sensitive & should be promptly delivered to the lab.

10 May

FLOW CYTOMETRY, ACUTE LEUKEMIA PANEL

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Flow Cytometry

Synonyms/Aliases

N/A

Abbreviations

N/A

^Type

Profile/ Panel (Bundled Tests).

^{5 Days}

Results ready Within 5 days.

^Method

Flow Cytometry Immunophenotyping

^Temp

Room Temperature (Avoid regrigeration)

^Setup

MON-FRI

Components Parameters

Variable markers to assess cell maturation status & lineage e.g. CD34, CD117, TDT (MARKERS OF IMMATURITY), CD3 (T CELL MARKER), PAX5, CD19/ CD20 (B CELL MARKERS), MPO (MYELOID MARKER) etc.

Pre-analytic Considerations

N/A

Test Ordering instructions

Patient preparation

N/A

Sample type

EDTA whole blood/ bone marrow plus Heparin whole blood/ bone marrow plus well prepared, labeled & fixed slides.

Container/ preservative

EDTA (PURPLE TOP) & HEPARIN (GREEN TOP) vacutainers. slides should be prepared from the EDTA vacutainer.

Sample volume

3ML Each

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted samples.

Other Instructions

N/A

Reference intervals

Negative.

Clinical Utility

Classification and diagnosis of Acute Leukemia.

Test Limitations/ Confounders

Samples are time sensitive & should be promptly delivered to the lab.

10 May

FILARIA MICROSCOPY

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Infectious

Synonyms/Aliases

N/A

Abbreviations

N/A

^Type

Individual Test.

^{24 Hrs}

Results ready within 24 Hours.

^Method

Smear Review Microscopy

^Temp

Room Temperature/Refrigerated.

^Setup

Daily

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly. Collection between 10pm-5am is recommended because species e.G. W. BANCROFTI exhibit Nocturnal Periodicity.

Patient preparation

N/A

Sample type

EDTA Whole Blood

Container/ preservative

EDTA (Purple Top) Vacutainer.

Sample volume

3ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample.

Other Instructions

N/A

Reference intervals

Negative for Microfilariae.

Clinical Utility

Evaluation of Lymphatic Filariasis.

Test Limitations/ Confounders

N/A

10 May

ERYTHROCYTE SEDIMENTATION RATE

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

General.

Synonyms/Aliases

SED Rate

Abbreviations

ESR

^Type

Individual Test.

^{2 Hrs}

Results ready in 2 Hours.

^Method

Westergren Method.

^Temp

Refrigerated (stability: 6 hours ambient; 24 hours refrigerated).

^Setup

Daily

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA Whole Blood

Container/ preservative

EDTA (Purple Top) Vacutainer.

Sample volume

3ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted sample; samples >24 hours old.

Other Instructions

N/A

Reference intervals

Age & gender appropriate values are provided on the report

Clinical Utility

Non-specific test used as a marker of Inflamation and Neoplasia.

Test Limitations/ Confounders

Anemia Increases Esr; Polycythemia, Sickle Cells Reduce Esr.

10 May

CELL COUNTS, FLUID (OTHER THAN CSF)

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Fluid Analysis

Synonyms/Aliases

Pleural Fluid Cell Count; Peritoneal/ Ascitic Fluid Cell Count; Synovial/ Joint Fluid Cell Count; Pericardial Fluid Cell Count.

Abbreviations

N/A

^Type

Profile/ Panel (Bundled Tests).

^{3 Hrs}

Results ready in 3 Hours

^Method

Microscopic Examination.

^Temp

2-8ºC

^Setup

Daily

Components Parameters

RBC cell count, WBC cell count (total & differentials); reporting of any foreign cells.

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

Variable i.e. Pleural Fluid, Peritoneal/ Ascitic Fluid; Synovial/ Joint Fluid etc.

Container/ preservative

EDTA (purple top) Vacutainer or plain sterile leak proof container.

Sample volume

2ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; clotted sample; insufficient sample; sample.

Other Instructions

N/A

Reference intervals

Fluid appropriate values are provided on the report.

Clinical Utility

Evaluation of Suspected CNS Pathology I.e. Infections (E.g. Meningitis), Malignancy (E.g. Leukemia), Demyelinating disease Etc.

Test Limitations/ Confounders

N/A

10 May

CD4 COUNT

May 10, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Flow Cytometry

Synonyms/Aliases

T-Helper Cells.

Abbreviations

CD4, T4 Cells

^Type

Individual Test.

^{5 days}

Results ready in 5 working days

^Method

Flow Cytometry Immunophenotyping

^Temp

Room temperature (avoid refrigeration)

^Setup

Daily

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA Whole Blood

Container/ preservative

EDTA (Purple Top) Vacutainer.

Sample volume

3ML

Rejection criteria

Labeling issue (unlabeled/ mislabeled); Missing request form; wrong sample type; Insufficient sample; Clotted sample.

Other Instructions

Time sensitive Analyte.

Reference intervals

Age & gender appropriate values are provided on the report

Clinical Utility

Assessment of immune status e.g. In patients with HIV.

Test Limitations/ Confounders

N/A

09 May

CELL COUNTS, CEREBROSPINAL FLUID

May 9, 2023May 10, 2023vincentochiengde

Category

Hematology

Sub Category

Fluid Analysis

Synonyms/Aliases

Cell Counts, CSF; Spinal Fluid Cell Counts.

Abbreviations

N/A

^Type

Profile/ Panel (Bundled Tests).

^{3 Hrs}

Results ready in 3 Hours

^Method

Microscopic Examination.

^Temp

2-8ºC

^Setup

Daily

Components Parameters

RBC cell count, WBC cell count (total & differentials); reporting of any foreign cells.

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

Cerebrospinal Fluid.

Container/ preservative

EDTA (purple top) Vacutainer or plain sterile leak proof container.

Sample volume

At least 0.5-1ml of CSF in sterile leak proof container. If multiple tests are required, minimum is 5ML divided between 3-4 sequentially labelled tubes.

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; clotted sample; insufficient sample; sample >24hours old.

Other Instructions

CSF should ideally be collected in multiple tubes labeled in accordance with the collection sequence. when this is done, the last tube drawn (tube 3/ 4) is used for cell counts.

Reference intervals

Age & gender appropriate values are provided on the report.

Clinical Utility

Evaluation of Suspected CNS Pathology I.e. Infections (E.g. Meningitis), Malignancy (E.g. Leukemia), Demyelinating disease Etc.

Test Limitations/ Confounders

Cell Lysis & distortion begins at 1 hour test should be handled as a stat (urgent) test; for meaningful results, prompt delivery should be done.

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