Haematology

EDTA (purple top) vacutainer; when reporting results, positive results must be speciated and quantified. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Ambient ANALYTE STABILITY Room Temperature: 1 day at 20-25°C │ Refrigerated: 7 days at 2-8°C

1) Liaise with lab team for booking of direct clients
2) Name & contact details of the referring doctor must be available
3) Previous patient records & test results, imaging inclusive to be availed
4) Recent CBC, <=72 hours old needed (concurrent CBC can be obtained at time of the procedure; PBF examination will still be required unless recently performed at Scanlab).
5) Collect a concurrent serum sample since other tests are frequently required.
6) For <12-14 year olds, anaesthesia is required at additional cost (for young children, may have to be done as an inpatient procedure)
Includes sample collections for PBF and BM aspirate PLUS trephine biopsy or clot section. For guidance on preparation of peripheral blood films, review this YouTube video: https://www.youtube.com/watch?v=kafqQZmrgQE For guidance on preparation of bone marrow aspirate films, review this YouTube video: https://www.youtube.com/watch?v=t_yOm3jaCCI For guidance on packaging of slides for transportation, review this YouTube video: https://www.youtube.com/watch?v=u-Vy80b_-sI&t=2s

1) Exact age (date of birth) required for accurate interpretation.
2) History of transfusion (or no transfusion) within the last 3-4 months required.
3) CBC/ red cell indices
4) EDTA (purple top) vacutainer
SHIPPING/ TRANSPORTATION REQUIREMENTS
Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 3 days at 20-25°C │ Refrigerated: 7 days at 2-8°C │ Frozen: 6 moths at -20°C

Labeled slides or <1 hr old sample in EDTA (purple top) vacutainer. For direct clients, under clinical history capture whether or not the patient has been transfused within the past 3-4 months. For guidance on preparation of peripheral blood films, review this YouTube video: https://www.youtube.com/watch?v=kafqQZmrgQE For guidance on packaging of slides for transportation, review this YouTube video: https://www.youtube.com/watch?v=u-Vy80b_-sI&t=2s

TEST ORDERING: Fill out test request form fully & legibly. CBC, concurrent PBF, referrer's contact information and detailed medical history are also required (bone marrow history form can be shared). Relevant history includes suspected diagnosis & any relevant investigation details (lab, imaging) as applicable. Specify the exact sample type(s) collected, anatomic site & laterality (e.g. left posterior superior iliac spine) and the fixative used. CONTAINER/ PRESERVATIVE Trephine biopsy in 10% neutral buffered formalin in a leak proof container OR if the biopsy is already processed, in formalin fixed paraffin embedded block (FFPE). GUIDANCE - YOUTUBE VIDEOS For preparation of peripheral blood films, review this YouTube video: https://www.youtube.com/watch?v=kafqQZmrgQE For preparation of 10% neutral buffered formalin, see this video: https://www.youtube.com/watch?v=Vr2y4-fAOs4

TEST ORDERING: Fill out test request form fully & legibly. CBC, concurrent PBF, referrer's contact information and detailed medical history are also required (bone marrow history form can be shared). Relevant history includes suspected diagnosis & any relevant investigation details (lab, imaging) as applicable. Specify the exact sample type(s) provided, anatomic site & laterality (e.g. left posterior superior iliac spine) and the fixative used. CONTAINER/ PRESERVATIVE Freshly prepared (<1 hour from collection), well spread, labeled bone marrow aspirate slides. Freshly prepared (<1 hour from collection), well spread, labeled peripheral blood film slides. GUIDANCE - YOUTUBE VIDEOS For preparation of peripheral blood films, review this video: https://www.youtube.com/watch?v=kafqQZmrgQE For preparation of bone marrow aspirate films, review this video: https://www.youtube.com/watch?v=t_yOm3jaCCI For packaging of slides for transportation, review this video: https://www.youtube.com/watch?v=u-Vy80b_-sI&t=2s

FROZEN, DOUBLE CENTRIFUGED CITRATED PLASMA required. Collect whole blood in citrate tube(s) filled up to the mark, centrifuge, separate plasma, centrifuge plasma, separate & FREEZE supernatant. Two 2 mL aliquots required. Detailed guidance on preparation of platelet poor (double centrifuged) plasma can be found here: https://www.youtube.com/watch?v=9z6c8Bgr2EY SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Frozen: 1 month at -20°C

EDTA whole blood, Citrated plasma. At collection citrate tube must be filled up to the mark. Test components APTT, PT/INR. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 2-8 hours at 20-25°C │ Refrigerated: 2-8 hours at 2-8°C │ Frozen: 2 WEEKS at -20°C

2mL frozen platelet poor citrated plasma. SAMPLE COLLECTION & HANDLING A. BLOOD COLLECTION Fill tube completely (to ensure proper 9:1 blood-to-citrate ratio). Gently invert 3-6 times (no vigorous shaking to prevent hemolysis). For UFH monitoring: Draw sample at steady-state (usually 4–6 hours after IV infusion starts). For LMWH monitoring: Draw peak level at 4 hours post-subcutaneous injection. For DOACs (rivaroxaban/apixaban): Timing depends on purpose (peak: ~2–4 hrs post-dose; trough: just before next dose). B. PROCESSING Centrifuge promptly (within 1 hour of collection). Separate plasma carefully (avoid platelet contamination). Freeze plasma. For instructions/ further guidance on how to prepare platelet poor plasma, please visit this YouTube video: https://www.youtube.com/watch?v=9z6c8Bgr2EY

EDTA (purple top) vacutainer. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Ambient ANALYTE STABILITY Room Temperature: 1 day at 20-25°C │ Refrigerated: 7 days at 2-8°C