12 May

URINE CULTURE & SENSITIVITY (AEROBIC)

vincentochiengde

Category

Microbiology

Sub Category

culture & sensitivity only (C/S)

Synonyms/Aliases

N / A

Abbreviations

Urine CS

Type

Profile/ panel (bundled tests)

Results

Results will be ready after 3 DAYS

Method

culture and antibiotic sensitivity testing (where indicated) using conventional methods

Temp

Transported at room temperature if promptly delivered; refrigerate (2-8ºc) if >2 hour delay before processing is expected.

Setup

Setup perfomed daily

Pre-analytic Considerations :

.

COMPONENTS/ PARAMETERS:

pathogen isolation, identification & reporting of appropriate antibiotics as applicable

Test ordering Instructions

fill out test request form fully & legibly. specify whenever urine is obtained by catheterization, via suprapubic aspirate or surgically.

Patient preparation

give instructions for collection of clean catch, midstream urine prior to collection.

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

urine, midstream specimen (MSSU); urine can also be obtained, as applicable, by in & out Catheterization, Suprapubic aspirate or surgically obtained e.g. Cystoscopy.

Container/ preservative

Sterile , Leak proof container

Sample volume

20 milliliters

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type/ preservative (samples containing boric acid preservative are inappropriate for dipstick examination)

Other Instructions

this test does not include microscopy & dipstick examination. if desired, please order urine microscopy, culture & sensitivity (urine m/c/s)

Reference intervals

sterile culture for surgically obtained specimens; no significant growth of uropathogens for mid stream specimen of urine (MSSU).

Clinical Utility

evaluating for urinary tract infection (UTI) - Cystitis, Pyelonephritis, Asymptomatic Bacteriuria etc.

Test Limitations/ Confounders

failure to collect mssu specimens can result in high culture contamination rates and requests for repeat collection. prior exposure to antibiotics can lead to false negative results.
11 May

URINALYSIS

vincentochiengde

Category

Microbiology

Sub Category

Fluid Analysis

Synonyms/Aliases

urine microscopy, urine dipstick examination, urine routine examination

Abbreviations

N/A

Type

Profile/ panel (bundled tests)

Results

Results will be ready after 3 hours

Method

macroscopic, dipstick & microscopic examination

Temp

Transported at room temperture if promptly delivered; refrigerate (2-8ºc) if >2 hour delay before processing is expected.

Setup

Setup perfomed daily

Pre-analytic Considerations :

.

COMPONENTS/ PARAMETERS:

Visual examination, dipstick, microscopy

Test ordering Instructions

Fill out request form fully & legibly.

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

spot urine. clean catch, midstream urine should be collected if culture is also required.

Container/ preservative

Sterile , Leak proof container

Sample volume

20 milliliters

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type/ preservative (samples containing boric acid preservative are inappropriate for dipstick examination)

Other Instructions

N/A

Reference intervals

Appropriate values are provided with report for the various parameters

Clinical Utility

evaluating for urine tract infection (UTI)

Test Limitations/ Confounders

N / A
11 May

STOOL ROTAVIRUS/ ADENOVIRUS ANTIGEN

vincentochiengde

Category

Microbiology

Sub Category

Antigen Test

Synonyms/Aliases

Rota / Adenovirus

Abbreviations

N/A

Type

Profile/ panel (bundled tests)

Results

Results will be ready after 3 hours

Method

immunochromatography

Temp

Transport temprature at room temperature. if >2hour delay is expected before delivery, refrigerate.

Setup

Setup perfomed daily

Pre-analytic Considerations :

.

COMPONENTS/ PARAMETERS:

Adenova / Rota

Test ordering Instructions

Fill out request form fully & legibly. Book with the lab/ confirm reagent availability.

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Stool

Container/ preservative

clean, dry, wide mouthed leak proof container which is labeled.

Sample volume

10 Grams

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type; empty sample containers; soiled diapers.

Other Instructions

Toilet paper is unsuitable for the collection.

Reference intervals

Negative

Clinical Utility

evaluation of rotavirus in patients with nosocomial diarrhoea, particularly infants, elderly and immunocompromised patients.

Test Limitations/ Confounders

N / A
11 May

STOOL MICROSCOPY

vincentochiengde

Category

Microbiology

Sub Category

Microscopy

Synonyms/Aliases

stool for ova/cyst

Abbreviations

Stool O/C

Type

profile / panel (bundled tests)

Results

Results will be ready after 2 -4 hours

Method

Microscopy

Temp

Transport temprature at room temperature. if >2hour delay is expected before delivery, refrigerate.

Setup

Setup perfomed daily

Pre-analytic Considerations :

.

COMPONENTS/ PARAMETERS:

Macroscopic & Microscopic examination of stool.

Test ordering Instructions

Fill out request form fully & legibly. Book with the lab/ confirm reagent availability.

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Stool

Container/ preservative

Clean, dry, wide mouthed leak proof container which is labeled.

Sample volume

10 Grams

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type; empty sample containers; soiled diapers.

Other Instructions

Sample can be collected directly into a clean wide mouth container. Alternatively if stool is solid/ semi-solid & the container lid has a spoon or spatula, collection can be done by scooping & transferring part of stool from a potty/ bedpan/ plastic bag (without touching the sides). Toilet paper is unsuitable for collection.

Reference intervals

specified on the report. depends on the parameter e.g. no blood, mucus, ova/cyst seen

Clinical Utility

work-up for Gastroenteritis & suspected parasitic infection.

Test Limitations/ Confounders

N / A
11 May

STOOL CULTURE & SENSITIVITY (AEROBIC)

vincentochiengde

Category

Microbiology

Sub Category

culture & sensitivity only (C/S)

Synonyms/Aliases

stool C/S

Abbreviations

N / A

Type

profile/ panel (bundled tests)

Results

Results will be ready after 3 days

Method

culture and antibiotic sensitivity testing (where indicated) using conventional methods

Temp

Transport temprature at room temperature. if >2hour delay is expected before delivery, refrigerate.

Setup

Setup perfomed daily

Pre-analytic Considerations :

.

COMPONENTS/ PARAMETERS:

microscopy, pathogen isolation, identification & reporting of appropriate antibiotics as applicable

Test ordering Instructions

Fill out request form fully & legibly. Book with the lab/ confirm reagent availability.

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Stool

Container/ preservative

clean, dry, wide mouthed container which is labeled. alternatively, transport swab in cary-blair transport medium.

Sample volume

10 Grams

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type; empty sample containers; soiled diapers.

Other Instructions

Toilet paper is unsuitable for collection.

Reference intervals

no growth obtained/ no pathogens isolated

Clinical Utility

Detecting bacteria responsible for infections.

Test Limitations/ Confounders

N / A
11 May

SPUTUM CULTURE & SENSITIVITY (AEROBIC)

vincentochiengde

Category

Microbiology

Sub Category

Microscopy/ culture/ sensitivity (M/C/S)

Synonyms/Aliases

sputum microscopy, culture & sensitivity

Abbreviations

Sputum C/S; Sputum M/C/S.

Type

profile/ panel (bundled tests)

Results

Results will be ready after 3 - 4 days

Method

culture and antibiotic sensitivity testing (where indicated) using conventional methods

Temp

Transport temprature at room temperature. refrigerate if delay is expected.

Setup

Setup perfomed daily

Pre-analytic Considerations :

.

Components / Parameters

microscopy, pathogen isolation, identification & reporting of appropriate antibiotics as applicable

Test ordering Instructions

Fill out request form fully & legibly. Book with the lab/ confirm reagent availability.

Patient preparation

rinse the mouth with water & cough deeply after taking deep breaths to get a good quality expectorated sputum specimen. collect sputum and not saliva.

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

sputum

Container/ preservative

Sterile Container

Sample volume

3 ML

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type; >25 squamous epithelial cells/ lpf on microscopy (indicative of saliva).

Other Instructions

if other tests are required e.g. tb culture, they must be separately ordered.

Reference intervals

Negative for Pathogens

Clinical Utility

detecting bacteria responsible for infections.

Test Limitations/ Confounders

prior exposure to antibiotics can result in false negative cultures; some potential pathogens may occur as normal flora in some patients.
11 May

SPERM WASHING

vincentochiengde

Category

Microbiology

Sub Category

Procedure

Synonyms/Aliases

Sperm Prepararion

Abbreviations

N / A

Type

N / A (Procedure)

Results

Results will be ready after 4 hours

Method

Density gradient centrifugation.

Temp

Room to body temperature

Setup

Setup perfomed on week days

Pre-analytic Considerations :

.

Components / Parameters

N / A

Test ordering Instructions

Fill out request form fully & legibly. Book with the lab/ confirm reagent availability.

Patient preparation

For walkin clients Only

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

semen

Container/ preservative

Sterile Containers

Sample volume

collect entirely volume

Rejection criteria

N / A

Other Instructions

Deliver promptly at room to body temperature. Collection time must be captured.

Reference intervals

N / A

Clinical Utility

To prepare semen samples for intrauterine insemination (IUI) or IVF

Test Limitations/ Confounders

N / A -
11 May

SEMEN ANALYSIS

vincentochiengde

Category

Microbiology

Sub Category

Fluid Analysis

Synonyms/Aliases

Semenalysis; Seminogram; Spermiogram; Spermogram.

Abbreviations

N / A

Type

Profile/ panel (bundled tests)

Results

Results will be ready after 4 hours

Method

Visual, chemical & microscopic examination in accordance with the guidelines contained in the latest who semenalysis manual.

Temp

Room to body temperature e.G. In inner pocket of a jacket.

Setup

Setup perfomed everyday

Pre-analytic Considerations :

.

Components / Parameters

Volume, color, vitality, motility, morphology, etc.

Test ordering Instructions

Fill out test request form fully & legibly. Clearly state indication I.E. Infertility/ subfertility assessment vs. Post vasectomy assessment.

Patient preparation

For walk-in clients only; must have abstained for a minimum of 48 hours & maximum of 7 days (if the indication is fertility assessment); must be at least 2-3 months post vasectomy & sexually active (if the indication is post vasectomy assessment). Detailed instructions available from the lab.

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

semen

Container/ preservative

Sterile Labeled Containers

Sample volume

collect sample entirely

Rejection criteria

Samples >1 hour old from time of collection.

Other Instructions

Must be delivered within 30 minutes of collection; collection time must be captured

Reference intervals

As per cut-offs provided in the latest version of the world health organization (who) semenalysis manual

Clinical Utility

Male (IN)fertility assessment; assessing the success of a vasectomy procedure.

Test Limitations/ Confounders

Samples collected in condoms may give erroneous values due to the presence of spermicides; incorrect abstinence (for fertility assessment) & post vasectomy periods may give rise to misleading results.
10 May

SALMONELLA ANTIGEN TEST

vincentochiengde

Category

Microbiology

Sub Category

Antigen Test

Synonyms/Aliases

N / A

Abbreviations

S A T

Type

Individual Tests

Results

Results will be ready after 2 hours

Method

Immunochromatography

Temp

Transport temprature kept at Room temperature (2 hours), 2-8ºc (1 day)

Setup

Setup perfomed everyday

Pre-analytic Considerations :

.

Components / Parameters

N / A

Test ordering Instructions

N / A

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Stool

Container/ preservative

Clean ,Leak Proof container

Sample volume

sample be at 10 Grams

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details. and wrong sample type

Other Instructions

N / A

Reference intervals

Negative

Clinical Utility

Diagnosis of Salmonela Infection

Test Limitations/ Confounders

N / A
10 May

ROUTINE MICROSCOPY AND GRAM STAIN

vincentochiengde

Category

Microbiology

Sub Category

Microscopy

Synonyms/Aliases

Gram Stain

Abbreviations

N/A -

Type

Individual Tests

Results

Results will be ready AFTER 1 HOUR

Method

Microscopy

Temp

Transport temprature kept BETWEEN 2-8ºC

Setup

Setup perfomed everyday

Pre-analytic Considerations :

.

Components / Parameters

N / A

Test ordering Instructions

N / A

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

variable including swabs & fluids

Container/ preservative

Sterile Container

Sample volume

2 ML

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details.

Other Instructions

If being transported refrigerate

Reference intervals

Negative (sterile sites)/ normal Flora only (non-sterile sites)

Clinical Utility

Identification of Gram positive and negative Organisms

Test Limitations/ Confounders

N / A
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