Routine chemistry

15ml of Spot Urine in a sterile leak-proof container. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Room Temperature: 45 DAYS at 20-25°C │ Refrigerated: 45 DAYS at 2-8°C │ Frozen: 12 MONTHS at -20°C

Serum in plain tube (red top) or SST tube (yellow top) or in a separation vial. Centrifuge tubes within 1 hour. Separate centrifuged serum in plain tubes within 1 hour. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 8 days at 20-25°C │ Refrigerated: 7 days at 2-8°C │ Frozen: 1 month at -20°C

Spot/ random urine in leak proof container. This test is prone to adulteration (dilution, swapping of samples etc.) by clients undergoing testing. Referring institutions should ensure that there is proper chain of custody. The lab reserves the right to take safeguards against adulteration e.g., requesting sample recollection, supervised collection etc.

15ml of Spot Urine in a sterile leak-proof container. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Room Temperature: 45 DAYS at 20-25°C │ Refrigerated: 2 months at 2-8°C │ Frozen: 1 year at -20°C

10ml aliquot of 24 hrs Urine in Sterile Urine Container

Serum in plain tube (red top) or SST tube (yellow top) or in a separation vial. Centrifuge tubes within 1 hour. Separate centrifuged serum in plain tubes within 1 hour.

TWO concurrently obtained sample types are required i.e. serum AND pleural fluid. On each of the sample types, protein and LDH are analysed and the pleural fluid:serum ratios of each analyte calculated. Pleural fluid total protein values should not exceed serum values (fluid LDH may sometimes exceed serum LDH; however QC should be checked and sample/ result transposition excluded). This test is to be performed in-house i.e. NOT outsourced

Serum in plain tube (red top) or SST tube (yellow top) or in a separation vial. Centrifuge tubes within 1 hour. Separate centrifuged serum in plain tubes within 1 hour. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 2 DAYS at 20-25°C │ Refrigerated: 7 DAYS at 2-8°C │ Frozen: 1 year at -20°C

Tourniquet use: Avoid tourniquet use (preferred) or apply the tourniquet briefly and release it before blood collection to avoid false elevation of calcium levels. Sample collection: Use a serum separator tube (SST) to collect the sample. Proper clotting: Allow the sample to clot fully at room temperature for about 30 minutes. Avoid hemolysis: Minimize trauma to the sample to prevent interference from hemolysis. Proper handling: Separate serum from cells within 1 hour of collection to prevent calcium binding. Sample volume: Ensure adequate sample volume for accurate analysis. Avoid air exposure to prevent changes in pH.