THERAPEUTIC VENESECTION

Category

Transfusion/ Immunohaematology

Sub Category

Transfusion/ Immunohaematology

Synonyms/Aliases

Therapeutic Phlebotomy

Abbreviations

N/A

Type

N/A (Procedure)

Results

Prior to the procedure.

Method

Venipuncture

Temp

Ambient/ Refrigerated.

Setup

Weekdays

Components Parameters

This is a procedure prescribed as part of medical treatment. To confirm eligibility for the procedure, a Hematocrit test will be performed prior. Ferritin test may be indicated in select patients.

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly; indication (diagnosis; reason) for the procedure must be stated. The amount to be removed, target Hematocrit value must be stated by the doctor.

Patient preparation

Therapeutic Phlebotomy consent must be filled.

Sample type

EDTA Whole Blood (For Hematocrit Assessment); Serum (For Ferritin Testing In Select Patients With a Diagnosis Of Iron Overload Or Hemochromatosis). Not Required If Recent Results Are Available.

Container/ preservative

Blood bags (for venesection); EDTA (purple top) vacutainer - for Hematocrit testing; plain top or SST (gel) - for Ferritin testing in select patients only.

Sample volume

3ML (Min 0.5ML) For Hematocrit Test; 2ML For Ferritin Test

Rejection criteria

Indication unknown; target Hematocrit value not specified.

Other Instructions

Procedure offered for walk-in clients only.

Reference intervals

Not Applicable (Procedure)

Clinical Utility

Patients with Polycythemia (primary or secondary).

Test Limitations/ Confounders

Patient must be in relatively stable condition to do the procedure as an outpatient e.g. Not in CCF.

INDIRECT COOMBS TEST (ICT)

Category

Transfusion/ Immunohaematology

Sub Category

Transfusion/ Immunohaematology

Synonyms/Aliases

Indirect Antiglobulin Test.

Abbreviations

ICT, IAT

Type

Individual Test.

3-4 Hrs

3-4 hours after receiving sample.

Method

Hemagglutination.

Temp

Room Temperature (24 Hours), Frozen (21 Days).

Setup

All working days.

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

Serum

Container/ preservative

SST, Red Top

Sample volume

4ML

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Wrong Sample Type; Insufficient Sample.

Other Instructions

N/A

Reference intervals

Negative.

Clinical Utility

Prenatal Antibody Screening, Autoimmune Hemolytic Anemia, Work Up Of Transfusion Reactions.

Test Limitations/ Confounders

N/A

DIRECT COOMBS TEST (DCT)

Category

Transfusion/ Immunohaematology

Sub Category

Transfusion/ Immunohaematology

Synonyms/Aliases

Direct Antiglobulin Test.

Abbreviations

DCT, DAT

Type

Individual test.

2-4 Hrs

2-4 hours after receiving sample.

Method

Hemagglutination Test.

Temp

Room Temperature (24 Hours); Refrigerated, 2 - 8⁰c (3 Days).

Setup

All working days.

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA whole blood.

Container/ preservative

EDTA (purple top) vacutainer.

Sample volume

2ML

Rejection criteria

Unlabeled/ Mislabeled Samples; Missing/ Wrong Request Form; Frozen Samples, Clotted Sample, Wrong Vacutainer Tube.

Other Instructions

Label sample with at least 2 patient identifiers e.g. Full name & date of birth.

Reference intervals

Negative.

Clinical Utility

Evaluation of autoimmune hemolytic Anemia, Hemolytic disease of the newborn.

Test Limitations/ Confounders

Presence of cold autoantibodies can affect interpretation of results, clotted sample can result to false positive results.

CONFIRMATORY BLOOD GROUP TESTING

Category

Transfusion/ Immunohaematology

Sub Category

Transfusion/ Immunohaematology

Synonyms/Aliases

ABO Group, Blood Type, RH Blood Group , ABO Typing, Blood Group

Abbreviations

RH, ABO

Type

Profile/ panel (bundled tests)

2-4 Hrs

2-4 hours after receiving sample.

Method

Hemagglutination.

Temp

Refrigerated.

Setup

All working days.

Components Parameters

ABO, Rhesus

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA whole blood.

Container/ preservative

EDTA (purple top) vacutainer.

Sample volume

3ML

Rejection criteria

Unlabeled/ Mislabeled Samples; Missing/ Wrong Request Form; Frozen Samples, Clotted Sample, Wrong Vacutainer Tube.

Other Instructions

Label sample with at least 2 patient identifiers e.g. Full name & date of birth.

Reference intervals

ABO Blood Group: A, B, Ab Or O; Rhesus D: Positive Or Negative.

Clinical Utility

Usually ordered to confirm blood group results obtained elsewhere [as part of pre-transfusion work-up; investigation of transfusion reactions; as part of blood or organ donor assessment; as part of profiles e.g. Antenatal (ANC) profile.

Test Limitations/ Confounders

Presence of cold autoantibodies can affect interpretation of results, clotted sample can result in false positive results.

BLOOD GROUP

Category

Transfusion/ Immunohaematology

Sub Category

Transfusion/ Immunohaematology

Synonyms/Aliases

ABO Group, Blood Type, Rhesus D Blood Group, ABO Typing

Abbreviations

RH, ABO

Type

Profile/ panel (bundled tests)

2-4 Hrs

2-4 hours after receiving sample.

Method

Hemagglutination Test.

Temp

Refrigerated.

Setup

All working days.

Components Parameters

ABO Blood Type; Rhesus D Blood Type

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

EDTA whole blood.

Container/ preservative

EDTA (purple top) vacutainer.

Sample volume

3ML (Min 0.5ML)

Rejection criteria

Unlabeled/ Mislabeled Samples; Missing/ Wrong Request Form; Frozen Samples, Clotted Sample, Wrong Vacutainer Tube.

Other Instructions

Label sample with at least 2 patient identifiers e.g. Full name & date of birth.

Reference intervals

ABO Blood Group: A, B, Ab Or O; Rhesus D: Positive Or Negative.

Clinical Utility

Pre-transfusion work-up; investigation of transfusion reactions; as part of blood or organ donor assessment; as part of profiles, e.g. Antenatal (ANC) profile.

Test Limitations/ Confounders

Presence of cold autoantibodies can affect interpretation of results, clotted sample can result in false positive results.