Category
SPECIAL CHEMISTRY.
Sub Category
ENDOCRINE
Synonyms/Aliases
CALCITRIOL; 1,25(OH)2 VITAMIN D; 1,25 DIHYDROXY CHOLECALCIFEROL.
Abbreviations
VIT D 1,25
Type
INDIVIDUAL TEST
7 Days
RESULTS READY/ REPORTED IN 5 WORKING DAYS.
Method
IMMUNOASSAY
Temp
REFRIGERATED (2℃-8℃).
Setup
BATCHED.
Components Parameters
NOT APPLICABLE.
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
FASTING PREFERRED. (NO SPECIAL PREPARATION REQUIRED)
Sample type
SERUM.
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
AT LEAST 2ML
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
AGE & GENDER APPROPRIATE VALUES ARE PROVIDED ON THE REPORT.
Clinical Utility
THIS TEST MEASURES THE BIOACTIVE FORM OF VITAMIN D. IT IS USED IN THE DIFFERENTIAL DIAGNOSIS OF HYPOCALCEMIA AND TO MONITOR PATIENTS WITH RENAL OSTEODYSTROPHY OR CHRONIC RENAL FAILURE.
Test Limitations/ Confounders
IMMUNOASSAY INTERFERENCE CAN GIVE RISE TO SPURIOUS RESULTS. SUCH INTERFERENCE CAN ARISE FROM PRESENCE OF HETEROPHILE ANTIBODIES, ANTI-ANIMAL ANTIBODIES, AUTOANTIBODIES, PARAPROTEINS, BIOTIN ETC. RESULTS SHOULD ALWAYS BE CORRELATED WITH CLINICAL FINDINGS.