Category
SPECIAL CHEMISTRY.
Sub Category
AUTOIMMUNE
Synonyms/Aliases
SCLERODERMA ANTIBODY; ANTI-TOPOISOMERASE ANTIBODY
Abbreviations
SCL-70; TOPO 1
Type
INDIVIDUAL TEST
Results
RESULTS READY/ REPORTED IN 7-10 WORKING DAYS
Method
NEPHELOMETRY
Temp
REFRIGERATED
Setup
BATCHED.
Components Parameters
NOT APPLICABLE
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. MENTION ANA FINDINGS IF AVAILABLE
Patient preparation
NO PREPARATION REQUIRED
Sample type
SERUM
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
AT LEAST 3ML
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
INTERPRETIVE DATA IS PROVIDED ON THE REPORT.
Clinical Utility
A FOLLOW-UP TEST TO A POSITIVE ANA WHERE THERE IS SUSPICTION FOR SCLERODERMA/ SYSTEMIC SCLEROSIS. CAN BE ORDERED SINGLY OR AS PART OF OTHER PANELS E.G. EXTRACTABLE NUCLEAR ANTIGEN (ENA) PANEL.
Test Limitations/ Confounders
UP TO 40% OF PATIENTS WITH SYSTEMIC SCLEROSIS CAN BE SERONEGATIVE I.E. NEGATIVE RESULTS DO NOT EXCLUDE SCLERODERMA. IF THERE IS A HIGH INDEX OF SUSPICION, CONSIDER ANTI-CENTROMERE, ANTI-RNA POLYMERASE 3 & ANTI PM-SCL