ANTI-STREPTOLYSIN O TITRE TEST (ASOT)

Sub Category

INFECTIOUS

Synonyms/Aliases

STREPTOCOCCAL ANTIBODIES, ANTI-HYALURONIDASE.

Abbreviations

ASOT; ASO.

^Type

INDIVIDUAL TEST.

^{2 HRS}

RESULTS READY IN 2 HRS.

^Method

QUANTITATIVE NEPHELOMETRY.

^Temp

AMBIENT (6 HOURS), REFRIGERATED (7 DAYS), FROZEN (14 DAYS)

^Setup

ALL WORKING DAYS.

Components Parameters

N/A

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM.

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

2 ML.

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.

Other Instructions

CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.

Reference intervals

NEGATIVE; TITRES ARE PROVIDED FOR POSITIVE CASES.

Clinical Utility

EVALUATE RECENT INFECTION WITH AND SEQUELAE OF GROUP A STREPTOCOCCI (GAS) I.E. RHEUMATIC FEVER & POST STREPTOCOCCAL GLOMERULONEPHRITIS.

Test Limitations/ Confounders

ANTIBODIES ARE DETECTED ≥3 WEEKS POST INFECTION.