Category
molecular genetic pathology & cytogenetics
Sub Category
cytogenetics
Synonym/Aliases
T(9;22), fish; philadelphia chromosome
Abbreviations
bcr::abl; bcr-abl; bcr/abl; t(9;22); t(9;22)(q34;q11)
Type
Individual Test
Results
Results ready in after 7 working days
Method
fluorescent in situ hybridization (fish)
Temp
Transportation Temprature be refrigirated (2-8ºC)
Setup
Batched
Components Parameters
N/A
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
Fill out test request form fully & legibly.
Patient preparation
N/A
Sample type
whole blood or bone marrow.
Container/ preservative
sodium heparin (dark green top) vacutainer.
Sample volume
3ml
Rejection criteria
labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample.
Other Instructions
avoid other types of heparin vacutainers e.g. lithium heparin
Reference intervals
Negative / Not Detected
Clinical Utility
detecting, quantifying and monitoring bcr::abl p190, p210 & p230 fusion transcripts. assessing cytogenetic response in patients with chronic myeloid leukemia (cml). detecting philadelphia positive acute lymphoblastic leukemia (all).
Test Limitations/ Confounders
it is less sensitive for detection of bcr::abl fusion transcripts than quantitative pcr in cml patients.