BCR::ABL, QUANTITATIVE PCR

Sub Category

oncology (hematologic malignancies)

Synonyms/Aliases

bcr::abl, quantitative pcr; philadelphia chromosome

Abbreviations

BCR::ABL; BCR-ABL; BCR/ABL; t(9;22)

^Type

Individual Test

^Results

Results ready in 3-5 working days

^Method

quantitative reverse transcription pcr. positive results for fusion transcripts are quantified as per the international scale (is) & instrument's linearity limit.

^Temp

Transportation Temprature be refrigirated (2-8ºC)

^Setup

Batched

Components Parameters

N/A; however concurrent CBC/ PBF ordering is required.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N/A

Sample type

edta whole blood or bone marrow.

Container/ preservative

edta (purple top) vacutainer

Sample volume

3ml

Rejection criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample.

Other Instructions

N/A

Reference intervals

Negative / Not Detected

Clinical Utility

detecting, quantifying and monitoring p210 abnormal fusion transcripts. assessing molecular response in patients with chronic myeloid leukemia (cml).

Test Limitations/ Confounders

it does not detect p190 (acute lymphoblastic leukemia) and p230 fusion transcripts.