Category
SEROLOGY/ IMMUNOLOGY
Sub Category
INFECTIOUS
Synonyms/Aliases
BRUCELLA ABORTUS AND BRUCELLA MELINTENSIS, BRUCELLA SEROLOGY.
Abbreviations
N/A
Type
PROFILE/ PANEL (BUNDLED TESTS).
2-4 Hrs
2-4 HOURS
Method
LATEX AGGLUTINATION.
Temp
AMBIENT (24 HOURS), REFRIGERATED (2 DAYS), FROZEN (7 DAYS).
Setup
ALL WORKING DAYS.
Components Parameters
B. ABORTUS SEROLOGY, B. MELITENSIS SEROLOGY
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
N/A
Sample type
SERUM.
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
2ML
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
NEGATIVE; <1:80 TITRES.
Clinical Utility
DIAGNOSIS OF BRUCELLOSIS/ UNDULANT FEVER/ MEDITERRANEAN FEVER. ≥FOUR-FOLD INCREASE IN TITRES BETWEEN ACUTE & CONVALESCENT SERA IS CONSISTENT WITH RECENT BRUCELLA INFECTION.
Test Limitations/ Confounders
FALSE POSITIVE RESULTS CAN OCCUR FROM OTHER INFECTIONS.