Category

SEROLOGY/ IMMUNOLOGY

Sub Category

INFECTIOUS

Synonyms/Aliases

BRUCELLA ABORTUS AND BRUCELLA MELINTENSIS, BRUCELLA SEROLOGY.

Abbreviations

N/A

Type

PROFILE/ PANEL (BUNDLED TESTS).

2-4 Hrs

2-4 HOURS

Method

LATEX AGGLUTINATION.

Temp

AMBIENT (24 HOURS), REFRIGERATED (2 DAYS), FROZEN (7 DAYS).

Setup

ALL WORKING DAYS.

Components Parameters

B. ABORTUS SEROLOGY, B. MELITENSIS SEROLOGY

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM.

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

2ML

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.

Other Instructions

CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.

Reference intervals

NEGATIVE; <1:80 TITRES.

Clinical Utility

DIAGNOSIS OF BRUCELLOSIS/ UNDULANT FEVER/ MEDITERRANEAN FEVER. ≥FOUR-FOLD INCREASE IN TITRES BETWEEN ACUTE & CONVALESCENT SERA IS CONSISTENT WITH RECENT BRUCELLA INFECTION.

Test Limitations/ Confounders

FALSE POSITIVE RESULTS CAN OCCUR FROM OTHER INFECTIONS.