FROZEN PREFERRED OR REFRIGERATED. AMBIENT (ROOM) TEMPERATURE NOT ACCEPTABLE.
Setup
DAILY
Components Parameters
NOT APPLICABLE
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. COLLECT BETWEEN 4-6 PM
Patient preparation
COLLECT BETWEEN 4-6 PM
Sample type
SERUM
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
AT LEAST 2ML.
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE. SAMPLE COLLECTED OUTSIDE THE SPECIFIED TIME
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
EVALUATION OF CUSHING'S SYNDROME, CUSHING'S DISEASE (HYPERCORTISOLISM) AND ADDISON'S DISEASE (HYPOCORTISOLISM).
Clinical Utility
EVALUATION OF CUSHING'S SYNDROME, CUSHING'S DISEASE (HYPERCORTISOLISM) AND ADDISON'S DISEASE (HYPOCORTISOLISM).
Test Limitations/ Confounders
USE OF STEROIDS AFFECTS RESULTS. IMMUNOASSAY INTERFERENCE CAN GIVE RISE TO SPURIOUS RESULTS. SUCH INTERFERENCE CAN ARISE FROM PRESENCE OF HETEROPHILE ANTIBODIES, ANTI-ANIMAL ANTIBODIES, AUTOANTIBODIES, PARAPROTEINS, BIOTIN ETC. RESULTS SHOULD ALWAYS BE CORRELATED WITH CLINICAL FINDINGS.