Category
SPECIAL CHEMISTRY.
Sub Category
ENDOCRINE
Synonyms/Aliases
OESTRADIOL; 17 BETA ESTRADIOL; OESTROGEN; ESTROGEN
Abbreviations
E2
Type
INDIVIDUAL TEST
Results
RESULTS READY/ REPORTED 24-48 HOURS
Method
IMMUNOASSAY.
Temp
AMBIENT, REFRIGERATED (PREFERRED) OR FROZEN.
Setup
DAILY
Components Parameters
NOT APPLICABLE
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
NO PREPARATION REQUIRED
Sample type
SERUM. HEPARIN PLASMA IS AN ACCEPTABLE ALTERNATE SPECIMEN.
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
AT LEAST 2 ML
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
AGE, GENDER, MENSTRUAL PHASE (FOLLICULAR PHASE VS. MID CYCLE PEAK VS. LUTEAL PHASE), POST MENOPAUSAL REFERENCE INTERVALS (AS APPROPRIATE) ARE PROVIDED ON THE REPORT.
Clinical Utility
ASSESSING OVARIAN FUNCTION, MONITOR FOLLICULAR DEVELOPMENT IN ASSISTED REPRODUCTION/ IVF PROTOCOLS, PRECOCIOUS PUBERTY, MENSTRUAL CYCLE (PERI-OVULATORY/ MID-CYCLE), GYNECOMASTIA.
Test Limitations/ Confounders
IMMUNOASSAY INTERFERENCE CAN GIVE RISE TO SPURIOUS RESULTS. SUCH INTERFERENCE CAN ARISE FROM PRESENCE OF HETEROPHILE ANTIBODIES, ANTI-ANIMAL ANTIBODIES, AUTOANTIBODIES, PARAPROTEINS, BIOTIN ETC. RESULTS SHOULD ALWAYS BE CORRELATED WITH CLINICAL FINDINGS.