Category
SEROLOGY/ IMMUNOLOGY
Sub Category
AUTOIMMUNE.
Synonyms/Aliases
ANTI-ENA.
Abbreviations
ENA
Type
PROFILE/ PANEL (BUNDLED TESTS).
7 DAYS
WITHIN 7 WORKING DAYS
Method
IMMUNOBLOT.
Temp
AMBIENT (48 HOURS), REFRIGERATED (7-14 DAYS), FROZEN 21 DAYS).
Setup
BATCHED.
Components Parameters
ANTI-SS-A (ANTI-Ro), ANTI-SS-B (ANTI-La), ANTI-SCL-70, ANTI-SM, ANTI-RNP, ANTI-JO-1.
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
N/A
Sample type
SERUM
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
2 ML.
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
NEGATIVE FOR EACH AUTO-ANTIBODY.
Clinical Utility
DIAGNOSE SYSTEMIC AUTOIMMUNE RHEUMATIC DISEASES E.G. SLE, MIXED CONNECTIVE TISSUE DISEASE (MCTD), SCLERODERMA, SJÖGREN'S SYNDROME, POLYMYOSITIS ETC. CAN BE DONE AS A FOLLOW-UP TEST FOR A POSITIVE ANA RESULT.
Test Limitations/ Confounders
N/A.