Category
Coagulation
SubCategory
bleeding/ bleeding risk assessment
Abbreviations
AHF
Synonyms/Aliases
Factor 8, Factor Viii Assay, Antihemophilic Factor, Hemophilia A, Anti-hemophilia A
Test
Individual Test
Results
results ready aftre 7-10 working Days
Method
chromogenic activity assay
Temp
Transportation temperature need to be Frozen
Setup
Setup is Conducted Once a week
Pre-analytic Considerations
.
Component Parameters
N/A
Sample Requirements
Citrated whole blood/ plasma
Test Ordering Instructions
fill out test request form fully & legibly.
Container / Presavative
Citrate (light blue top) vacutainer during sample collection.
Test Considerations
fill out test request form fully & legibly.
Patient Preparations
fasting preferred to avoid lipemia.
Sample Types
citrated platelet poor plasma (frozen)
Sample Volume
during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.
Rejection Criteria
labeling issue (unlabeled/ mislabeled); missing request form; wrong sample type; insufficient sample; hemolyzed samples; underfilled tubes, clotted samples, lipemic samples.
Reference intervals
Appropriate values are provided on the report
Clinical utility
Examination of prolonged APTT, diagnosis of VWF deficiency, diagnosis of Hemophilia A
Test Limitations /Confounders
this is a temperature sensitive analyte; frozen handling required.
Other Instructions
To prepare platelet poor plasma, double centrifuge sample immediately after collection i.e. centrifuge, transfer plasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.