Category

SEROLOGY/ IMMUNOLOGY

Sub Category

INFECTIOUS.

Synonyms/Aliases

N/A

Abbreviations

N/A

Type

INDIVIDUAL TEST.

5 DAYS

5 WORKING DAYS

Method

IMMUNOASSAY.

Temp

AMBIENT (7 DAYS), REFRIGERATED (14 DAYS), FROZEN (30 DAYS).

Setup

BATCHED.

Components Parameters

N/A

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

2 ML.

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.

Other Instructions

CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.

Reference intervals

NEGATIVE.

Clinical Utility

EVALUATION OF LYMPHEDEMA. DIAGNOSE EXPOSURE TO LYMPHATIC FILARIASIS/ ELEPHANTIASIS/ WUCHERERIA BANCROFTI INFECTION/ BRUGIA MALAYI INFECTION. ALSO CONSIDER EXAMINING PERIPHERAL BLOOD (COLLECTED AT MIDNIGHT FOR SUSPECTED W. BACROFTI & B. MALAYI) FOR MICROFILARIAE AS WELL AS FILARIA ANTIGEN TESTING.

Test Limitations/ Confounders

DOES NOT DISTINGUISH BETWEEN PAST & CURRENT ACTIVE INFECTION. FALSE POSITIVES CAN OCCUR FROM CROSS-REACTING ANTIBODIES TO OTHER HELMINTHS/ NEMATODES E.G. STRONGYLOIDES STERCORALIS. FALSE NEGATIVES CAN OCCUR.