Variable markers to assess cell maturation status & lineage e.g. CD34, CD117, TDT (MARKERS OF IMMATURITY), CD3 (T CELL MARKER), PAX5, CD19/ CD20 (B CELL MARKERS), MPO (MYELOID MARKER) etc.
Pre-analytic Considerations
N/A
Test Ordering instructions
Fill out test request form fully & legibly. Clinical history, CBC, PBF report findings must be provided. Sample type must be specified, I.E. Peripheral blood vs. Bone marrow. The specific panel required (Acute Leukemia vs. Chronic Leukemia/ Lymphoma) must be specified based on outcome of baseline investigations.
Patient preparation
N/A
Sample type
EDTA whole blood/ bone marrow plus Heparin whole blood/ bone marrow plus well prepared, labeled & fixed slides.
Container/ preservative
EDTA (PURPLE TOP) & HEPARIN (GREEN TOP) vacutainers. slides should be prepared from the EDTA vacutainer.