Category
Hematology
Sub Category
Flow Cytometry
Synonyms/Aliases
N/A
Abbreviations
N/A
Type
Profile/ Panel (Bundled Tests).
5 Days
Results ready Within 5 days.
Method
Flow Cytometry Immunophenotyping
Temp
Room Temperature (Avoid regrigeration)
Setup
MON-FRI
Components Parameters
Variable markers to assess cell maturation status & lineage e.g. CD34, CD117, TDT (MARKERS OF IMMATURITY), CD3 (T CELL MARKER), PAX5, CD19/ CD20 (B CELL MARKERS), MPO (MYELOID MARKER) etc.
Pre-analytic Considerations
N/A
Test Ordering instructions
Fill out test request form fully & legibly. Clinical history, CBC, PBF report findings must be provided. Sample type must be specified, I.E. Peripheral blood vs. Bone marrow. The specific panel required (Acute Leukemia vs. Chronic Leukemia/ Lymphoma) must be specified based on outcome of baseline investigations.
Patient preparation
N/A
Sample type
EDTA whole blood/ bone marrow plus Heparin whole blood/ bone marrow plus well prepared, labeled & fixed slides.
Container/ preservative
EDTA (PURPLE TOP) & HEPARIN (GREEN TOP) vacutainers. slides should be prepared from the EDTA vacutainer.
Sample volume
3ML Each
Rejection criteria
Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted samples.
Other Instructions
N/A
Reference intervals
Negative.
Clinical Utility
Classification and diagnosis of Acute Leukemia.
Test Limitations/ Confounders
Samples are time sensitive & should be promptly delivered to the lab.