Category

Hematology

Sub Category

Flow Cytometry

Synonyms/Aliases

N/A

Abbreviations

N/A

Type

Profile/ Panel (Bundled Tests).

5 Days

Results ready Within 5 days.

Method

Flow Cytometry Immunophenotyping

Temp

Room Temperature (Avoid regrigeration)

Setup

MON-FRI

Components Parameters

Variable markers to assess clonality & lineage e.g. KAPPA, LAMBDA (MARKERS OF CLONALITY), CD3 (T CELL MARKER), CD19/ CD20 (B CELL MARKERS) etc.

Pre-analytic Considerations

N/A

Test Ordering instructions

Fill out test request form fully & legibly. Clinical history, CBC, PBF report findings must be provided. Sample type must be specified, I.E. Peripheral blood vs. Bone marrow. The specific panel required (Acute Leukemia vs. Chronic Leukemia/ Lymphoma) must be specified based on outcome of baseline investigations.

Patient preparation

N/A

Sample type

EDTA whole blood/ bone marrow plus Heparin whole blood/ bone marrow plus well prepared, labeled & fixed slides.

Container/ preservative

EDTA (PURPLE TOP) & HEPARIN (GREEN TOP) vacutainers. slides should be prepared from the EDTA vacutainer.

Sample volume

3ML Each

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted samples.

Other Instructions

N/A

Reference intervals

Negative.

Clinical Utility

Classification and diagnosis of Chronic Leukemia/ Lymphoma.

Test Limitations/ Confounders

Samples are time sensitive & should be promptly delivered to the lab.