Category
Molecular Genetic Pathology & Cytogenetics
Sub Category
Infectious Diseases
Synonym/Aliases
Genexpert, Tb/rif Pcr; Genexpert Tb/rif Ultra Pcr
Abbreviations
TB PCR; TB/RIF PCR
Type
Profile/ Panel (Bundled Tests)
Results
RESULTS READY WITHIN 24-48 HOURS.
Method
AUTOMATED REAL TIME PCR
Temp
ROOM TEMPERATURE (3 DAYS), 2-8ºC (7 DAYS).
Setup
BATCHED
Components Parameters
M. Tuberculosis Complex, Rifampicin Resistance As Applicable.
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. SPECIFY EXACT SAMPLE TYPE.
Patient preparation
PATIENT INSTRUCTIONS SHOULD BE PROVIDED FOR SPUTUM COLLECTION (SALIVA SHOULD NOT BE SUBMITTED)
Sample type
VARIABLE DEPENDING ON SUSPECTED SITE OF INFECTION E.G. PULMONARY (SPUTUM, BRONCHOALVEOLAR LAVAGE. STOOL OR GASTRIC ASPIRATE FOR <5 YEAR OLDS UNABLE TO EXPECTORATE SPUTUM); PLEURAL (PLEURAL BIOPSY, PLEURAL FLUID); GASTROINTESTINAL (ASCITIC/ PERITONEAL FLUID, TISSUE BIOPSY), LYMPH NODES (BIOPSY OR ASPIRATE), CENTRAL NERVOUS SYSTEM (CSF), MUSCULOSKELETAL (SYNOVIAL FLUID, PUS, TISSUE) ETC.
Container/ preservative
STERILE, LEAK PROOF, PLASTIC CONTAINER
Sample volume
3 ML/ 1x1CM TISSUE
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; BLOOD STAINED SAMPLE.
Other Instructions
- N/A
Reference intervals
NOT DETECTED/ NEGATIVE FOR MYCOBACTERIUM TUBERCULOSIS.
Clinical Utility
DETECTION OF MYCOBACTERIUM TUBERCULOSIS & RIFAMPICIN RESISTANCE. THE LATTER IS USED AS AN INDICATOR OF POSSIBLE MULTI DRUG RESISTANT TUBERCULOSIS.
Test Limitations/ Confounders
- N/A