Category
Hematology
Sub Category
Haemolytic Anemia
Synonyms/Aliases
HB Electrophoresis; Abnormal Hemoglobin Studies
Abbreviations
HPLC
Type
Profile/ Panel (Bundled Tests)
7 days
Results ready in 7 days
Method
Electrophoresis or high performance liquid chromatography (hPLC).
Temp
Room temperature.
Setup
Daily
Components Parameters
HBF, HBA0, HBA2 quantification & detection of abnormal Hemoglobin variants.
Pre-analytic Considerations
N/A
Test Ordering Instructions
Fill out test request form fully & legibly. Exact age (date of birth preferred) and history of blood transfusion (within the past 3-4 months) is required.
Patient preparation
N/A
Sample type
EDTA whole blood.
Container/ preservative
EDTA (Purple Top) Vacutainer.
Sample volume
3ML
Rejection criteria
Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; clotted sample.
Other Instructions
N/A
Reference intervals
Age appropriate values provided with report.
Clinical Utility
HBAA with normal HBF & HBA2 levels for age.
Test Limitations/ Confounders
Recent transfusion is a confounder, therefore testing should be preferentially done >3-4 months post transfusion.