Category
Molecular Genetic Pathology & Cytogenetics
Sub Category
Infectious Diseases
Synonym/Aliases
Hbv Dna Quantitation; Hbv Quantitative PCR
Abbreviations
HBV DNA; HBV VL
Type
Individual Test
Results
RESULTS READY WITHIN 10 DAYS
Method
PCR
Temp
REFRIGERATED, 2-8ºC (7 DAYS); FROZEN (>30 DAYS)
Setup
BATCHED
Components Parameters
- N/A
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. SPECIFY SAMPLE TYPE.
Patient preparation
- N/A
Sample type
VARIABLE E.G. EDTA PLASMA/ SERUM
Container/ preservative
EDTA PLASMA IN SEPARATION VIAL, EDTA WHOLE BLOOD IN PLASMA SEPARATION TUBE (PST), SERUM SEPARATOR TUBE (SST).
Sample volume
5 ML EDTA WHOLE BLOOD OR 2ML EDTA PLASMA/ SERUM
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE (E.G. HEPARIN); INSUFFICIENT SAMPLE.
Other Instructions
- N/A
Reference intervals
NOT DETECTED/ NEGATIVE
Clinical Utility
MONITOR VIRAL LOAD & TREATMENT RESPONSE IN CHRONIC HBV INFECTION
Test Limitations/ Confounders
PRESENCE OF PCR INHIBITORS IN THE SAMPLE MAY PREVENT DNA AMPLIFICATION. PARADOXICAL RESULTS MAY ARISE DUE TO SELECTION OF INAPPROPRIATE SPECIMEN AND CONTAMINATION DURING SPECIMEN COLLECTION