Category

Anatomic Pathology

Sub Category

Surgical/ Histopathology

Synonyms/Aliases

Histopathology Consultation, Wet/ Unprocessed Tissue; Surgical Pathology Consultation, Wet/ Unprocessed Tissue.

Abbreviations

N/A

Type

Individual Test

14 Days

Results Ready in 14 Days

Method

Grossing, Tissue Processing, Staining And Microscopy

Temp

Room temperature; refrigerated is also acceptable.

Setup

Mon-Fri

Components Parameters

N/A

Pre-analytic Considerations

N/A

Test Ordering Instructions

Fill out test request form fully & legibly. Provide your contact information & complete history including suspected diagnosis & relevant investigation details (lab, imaging). Specify the exact sample type, procedure done, anatomic site, laterality where applicable (e.g. Left vs right) and the fixative used. Time of insertion into fixative is required for Mastectomy specimens.

Patient preparation

N/A

Sample type

A Variety Of Specimens Are Categorized As "Large" Based On Hybrid Criteria (Size/ Complexity/ Cost Of Processing/ Pathologist Time) E.g. Whole Organ(S), Large Amputation, Or A Large Biopsy Of Part Of An Organ. Examples: Prostatectomy, Mastectomy, Colectomy, Esophagectomy, Gastrectomy, Extremity (Limb) Amputation Or Disarticulation, Nephrectomy, Laryngectomy, Hepatectomy, Lung Resection, Splenectomy, Hysterectomy (With Or Without Adnexae), Lung (Segment, Lobar Or Total) Resection, Pancreas, Vulvectomy Specimens. Also, Soft Tissue Lesions ≥5 cm In Maximum Dimension, Bone Resections For Neoplasms, Eye Enucleation Or Exenteration, Gall Bladder & Porta Hepatis Radical Resection Etc.

Container/ preservative

10% formalin (10% neutral buffered formalin preferred) in appropriate size, leak proof container. Immerse specimen into formalin within 30 minutes of removal.

Sample volume

Volume of formalin to tissue of ≥10:1

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled Blocks); Missing Request Form; Request Form Not Accompanied By Sample; Mismatch Between Request Form & Sample Details; Missing Age/ Gender; Wrong Sample Type; Incorrect Preservative; Insufficient Sample; Empty Sample Containers.

Other Instructions

Incorrect preservative (e.g. Placing sample for Histology in normal saline) is likely to resut in sample Deterioration/ Putrefaction, Suboptimal evaluation & inability to give a diagnosis.

Reference intervals

Negative for disease / Malignancy

Clinical Utility

Provides diagnosis for diseases, from benign to malignant.

Test Limitations/ Confounders

Poorly fixed tissue