Grossing, Tissue Processing, Staining And Microscopy
Temp
Room temperature; refrigerated is also acceptable.
Setup
Mon-Fri
Components Parameters
N/A
Pre-analytic Considerations
N/A
Test Ordering Instructions
Fill Out Test Request Form Fully & Legibly. Provide Your Contact Information & Complete History Including Suspected Diagnosis & Relevant Investigation Details (Lab, Imaging). Specify The Exact Sample Type, Procedure Done, Anatomic Site, Laterality Where Applicable (E.g. Left Vs Right) And The Fixative Used.
Patient preparation
N/A
Sample type
A Variety Of Specimens Are Categorized As "Small" Based On Hybrid Criteria (Size/ Complexity/ Cost Of Processing/ Pathologist Time) E.g. Appendix, Bone Biopsy Or Curettings (Bone Resections For Malignancy Are Large Specimens), Branchial Cleft Or Cyst, Breast Biopsy (Mastectomy Specimens Are Large Specimens), Bronchial Biopsy, Cervix Biopsy, Cholesteatoma, Colon Biopsy, Conjunctival Biopsy Or Pterygium, Cornea, Endometrial Curettings, Endoscopic Biopsy, Fallopian Tube, Gall Bladder, Ganglion Cyst, Gastric Biopsy, Gum Or Oral Mucosa Biopsy, Hernia Sac, Hydrocele Sac, Joint & Periarticular Tissue Without Bone, Laryngeal Biopsy, Lung (Needle Or Transbronchial) Biopsy, Lymph Node Biopsy, Nasopharyngeal Biopsy, Oesophageal Biopsy Or Diverticulum, Omentum Biopsy, Oropharyngeal Biopsy, Peritoneal Biopsy, Pleural Or Pericardial Biopsy/ Tissue; Products Of Conception (POCs), Rectal Biopsy, Skin Biopsy, Small Bowel Biopsy, Testis, Testis Biopsy, Thyroglossal Duct, Tongue Biopsy (Tongue With Local Neoplasm Or With Nodes Is Categorized As A Medium Specimen), Tracheal Biopsy, Tonsil Or Adenoids, Ureter Biopsy Or Resection, Urethra Biopsy Or Resection, Vaginal Biopsy Etc.
Container/ preservative
10% Formalin (10% Neutral Buffered Formalin Preferred) In Appropriate Size, Leak Proof Container. Immerse Specimen Into Formalin Within 30 Minutes Of Removal. Ensure Specimen Is Immersed In Fixative & Not Stuck On The Side/ Lid Of The Container.
Sample volume
Volume of formalin to tissue of ≥10:1
Rejection criteria
Labeling Issue (Unlabeled/ Mislabeled Blocks); Missing Request Form; Request Form Not Accompanied By Sample; Mismatch Between Request Form & Sample Details; Missing Age/ Gender; Wrong Sample Type; Incorrect Preservative; Insufficient Sample; Empty Sample Containers.
Other Instructions
Incorrect preservative (e.g. Placing sample for Histology in normal saline) is likely to resut in sample Deterioration/ Putrefaction, Suboptimal evaluation & inability to give a diagnosis.