HIV 1 PROVIRAL DNA (QUALITATIVE PCR)

Sub Category

Infectious Disease

Synonym/Aliases

- N/A

Abbreviations

- N/A

^Type

Individual Test

^Results

RESULTS READY WITHIN 5 DAYS

^Method

REAL TIME PCR

^Temp

ROOM TEMPERATURE 2 DAYS, 2-8ºC

^Setup

BATCHED

Components Parameters

- N/A

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY

Patient preparation

- N/A

Sample type

EDTA WHOLE BLOOD

Container/ preservative

FORMALIN FIXED, PARAFFIN EMBEDDED BLOCKS [PREFERRED]. ALTERNATIVELY, 4-5 MICRONS UNSTAINED SECTIONS ON POSITIVELY-CHARGED SLIDES (≥FOUR SLIDES).

Sample volume

3 ML

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.

Other Instructions

- N/A

Reference intervals

NEGATIVE

Clinical Utility

PRIMARILY USED IN EARLY INFANT DIAGNOSIS (EID) OF HIV-1 INFECTION I.E. DIAGNOSING HIV 1 INFECTION IN HIV-1 EXPOSED CHILDREN AGED 18 MONTHS (BORN TO HIV 1 POSITIVE MOTHERS).

Test Limitations/ Confounders

PRESENCE OF PCR INHIBITORS IN THE SAMPLE MAY PREVENT NUCLEIC ACID AMPLIFICATION. PARADOXICAL RESULTS MAY ARISE DUE TO SELECTION OF INAPPROPRIATE SPECIMEN AND CONTAMINATION DURING SPECIMEN COLLECTION.