Category
Molecular Genetic Pathology & Cytogenetics
Sub Category
Infectious Disease
Synonym/Aliases
Hiv-1 Rna Detection
Abbreviations
- N/A
Type
Individual Test
Results
RESULTS READY WITHIN 7 DAYS
Method
REAL TIME PCR
Temp
REFRIGERATED (2-8ºC) OR FROZEN (-20ºC) EDTA PLASMA.
Setup
BATCHED
Components Parameters
- N/A
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. SPECIFY SAMPLE TYPE
Patient preparation
- N/A
Sample type
EDTA PLASMA (CENTRIFUGE & SEPARATE PLASMA FROM WHOLE BLOOD WITHIN 2 HOURS.
Container/ preservative
EDTA
Sample volume
2-3 ML EDTA PLASMA
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.
Other Instructions
- N/A
Reference intervals
NOT DETECTED/ NEGATIVE
Clinical Utility
DIAGNOSIS OF HIV 1 INFECTION
Test Limitations/ Confounders
PRESENCE OF PCR INHIBITORS IN THE SAMPLE MAY PREVENT NUCLEIC ACID AMPLIFICATION. PARADOXICAL RESULTS MAY ARISE DUE TO SELECTION OF INAPPROPRIATE SPECIMEN AND CONTAMINATION DURING SPECIMEN COLLECTION.