HPV DNA DETECTION & TYPING

Sub Category

Infectious Diseases

Synonym/Aliases

Human Papilloma Virus Detection & Typing; High Risk Hpv.

Abbreviations

HPV DNA

^Type

Profile/ Panel (Bundled Tests)

^Results

RESULTS READY WITHIN 10 DAYS

^Method

PCR

^Temp

ROOM TEMPERATURE (14 DAYS), 2-8ºC (14 DAYS)

^Setup

BATCHED

Components Parameters

Testing For Multiple High Risk Hpv Types.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. SPECIFY EXACT SPECIMEN TYPE/ SOURCE.

Patient preparation

IN CASE OF SELF SAMPLING, MANUFACTURER'S INSTRUCTIONS FOR SELF COLLECTED SPECIMENS APPLY.

Sample type

VARIABLE I.E. SWABS (E.G. VAGINAL, ENDOCERVICAL, RECTAL), CERVICAL CELLS IN LIQUID BASED CYTOLOGY (LBC) VIAL OR OTHER ACCEPTABLE MEDIA ETC. (REFER TO MANUFACTURER'S INSTRUCTIONS IF IN DOUBT).

Container/ preservative

ENTIRE LBC VIAL OR APPROPRIATE SWAB IN TRANSPORT TUBE.

Sample volume

- N/A

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.

Other Instructions

- N/A

Reference intervals

NEGATIVE FOR SPECIFIED HIGH RISK GENOTYPES E.G. 16, 18 ETC.

Clinical Utility

HPV IS A COMMON SEXUALLY TRANSMITTED INFECTION (STI). HIGH RISK HPV GENOTYPES (E.G. 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) ARE ASSOCIATED WITH DYSPLASIA (PRE-MALIGNANT) & NEOPLASIA (CANCER) OF THE CERVIX. THEY ARE ALSO ASSOCIATED WITH OTHER MALIGNANCIES E.G. SOME PENILE, ANAL CANCERS ETC. HPV DETECTION IS A SCREENING STRATEGY FOR CERVICAL CANCER.

Test Limitations/ Confounders

PRESENCE OF PCR INHIBITORS IN THE SAMPLE PREVENTS DNA AMPLIFICATION FOR HPV DETECTION.