Category
SEROLOGY/ IMMUNOLOGY
Sub Category
INFECTIOUS.
Synonyms/Aliases
HIV p24 ANTIGEN/ ANTIBODY TEST, ELISA; HIV Ag/ Ab SEROLOGY; HIV 1/2 SEROLOGY.
Abbreviations
HIV ELISA, HIV DUO, HIV COMBO.
Type
INDIVIDUAL TEST.
5 DAYS
5 WORKING DAYS.
Method
AUTOMATED IMMUNOASSAY.
Temp
AMBIENT (24 HOURS), REFRIGERATED (7 DAYS), FROZEN (14 DAYS).
Setup
BATCHED.
Components Parameters
N/A
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
N/A
Sample type
SERUM.
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
2 ML.
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
NEGATIVE (POSITIVE RESULTS ARE RECHECKED BY A DIFFERENT METHOD/ KIT IN LINE WITH NASCOP HTS ALGORITHM).
Clinical Utility
A POSITIVE RESULT IS CONSISTENT WITH CURRENT/ RECENT HSV 2 INFECTION. IgM IS THE FIRST ANTIBODY PRODUCED BY THE IMMUNE SYSTEM IN RESPONSE TO INFECTION.
Test Limitations/ Confounders
N/A.