Category
Molecular Genetic Pathology & Cytogenetics
Sub Category
Cytogenetics
Synonym/Aliases
N/A
Abbreviations
N/A
Type
Individual Test
Results
RESULTS READY WITHIN 18 - 21 DAYS
Method
CHROMOSOMAL ANALYSIS VIA CELL CULTURE, EXPOSURE TO MITOGENS, BANDED KARYOGRAMS & MICROSCOPIC ANALYSIS OF METAPHASE CELLS.
Temp
REFRIGERATE. TIME SENSITIVE THREFORE NOTIFY LAB PRIOR TO COLLECTION & PROMPTLY DELIVER SAMPLE TO THE LAB.
Setup
BATCHED
Components Parameters
- N/A
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. PROVIDE CLINICAL HISTORY. NOTIFY THE LAB PRIOR TO COLLECTION. CONSIDER CHROMOSOMAL MICROARRAY (CMA) AS A POSSIBLE ALTERNATIVE TEST.
Patient preparation
IN CASE OF SELF SAMPLING, MANUFACTURER'S INSTRUCTIONS FOR SELF COLLECTED SPECIMENS APPLY.
Sample type
VIABLE FETAL TISSUE/ PRODUCTS OF CONCEPTION (POCs) IN TISSUE CULTURE MEDIA (OR RPMI/ HANK'S/ RINGER'S LACTATE/ NORMAL SALINE TO WHICH 2-3 DROPS OF GENTAMICIN HAVE BEEN ADDED). FIRST TRIMESTER LOSS: POCs IN CULTURE MEDIA OR NORMAL SALINE WITH ANTIBIOTICS. SECOND TRIMESTER AND BEYOND: DUAL SPECIMENS PREFERRED I.E. 1) PLACENTAL BIOPSY FROM FETAL SIDE WITHIN 1 INCH OF THE PLACENTAL INSERTION SITE PLUS 2) FETAL TISSUE (E.G. MUSCLE BIOPSY FROM MEDIAL ASPECT OF THE THIGH OR FETAL TOE).
Container/ preservative
TISSUE BIOPSY IN STERILE CONTAINER CONTAINING TISSUE CULTURE MEDIUM (OR RPMI/ HANK'S/ RINGER'S LACTATE/ NORMAL SALINE TO WHICH 2-3 DROPS OF GENTAMICIN HAVE BEEN ADDED).
Sample volume
EACH BIOPSY SHOULD BE AT LEAST ~5x5 MM (~SIZE OF PENCIL RUBBER/ ERASER) OR LARGER.
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; FROZEN SAMPLE.
Other Instructions
IDENTIFY SAMPLES FROM TWINS UNIQUELY WHERE APPLICABLE.
Reference intervals
INTERPRETIVE DATA IS PROVIDED ON THE REPORT.
Clinical Utility
ESTABLISHING CAUSES OF PREGNANCY LOSSES & FETAL ABNORMALITIES ARISING FROM DETECTS NUMERICAL & STRUCTURAL CHROMOSOMAL ABNORMALITIES WITHIN THE ASSAY'S LIMIT OF DETECTION.
Test Limitations/ Confounders
A NORMAL RESULT DOES NOT COMPLETELY EXCLUDE GENETIC DISORDERS E.G. SINGLE GENE DISORDERS, SUBMICROSCOPIC CYTOGENETIC ABNORMALITIES BELOW THE ASSAY'S DETECTION LIMIT.