Category
Anatomic Pathology
Sub Category
Surgical/ Histopathology
Synonyms/Aliases
renal biopsy immunofluorescence stain
Abbreviations
IF
Type
Individual Test
Result
Results will be ready after 14 days
Method
Immunofluorescence Stain & Reporting
Temp
Transported temprature be at room temperature; refrigerated is also acceptable.
Setup
Mon-Fri All working Days
Components Parameters
N/A
PRE-ANALYTIC CONSIDERATIONS
:
Test Ordering Instructions
Fill out test request form fully & legibly. provide your contact information & complete history. h&e report required.
Patient preparation
N/A
Sample type
Renal biopsy immediately placed in michel's medium/ zeus fixative. confirm that the specimen is immersed in the fixative. label should contain at least 2 patient identifiers, sample & fixative type.
Container/ preservative
Renal biopsy immediately placed in michel's medium/ zeus fixative. confirm that the specimen is immersed in the fixative. label should contain at least 2 patient identifiers, sample & fixative type.
Sample volume
specimen must contain Glomeruli at assessment.
Rejection criteria
Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; wrong sample type or fixative (formalin fixed samples will be rejected for if studies); empty sample containers.
Other Instructions
This is not a standalone diagnostic test; it should be ordered alongside light microscopy (histopathology examination, h&e stain); electron microscopy, em studies may occasionally be required (samples for em should be in glutaraldehyde as fixative). at collection, aim for ≥3 biopsy specimens & place one in 10% neutral buffered formalin, another in michel's medium & the other in glutaraldehyde. if glomeruli are found to be absent, if studies will be inconclusive.
Reference intervals
N/A
Clinical Utility
Diagnostic or confirmatory
Test Limitations/ Confounders
N/A