Category
SPECIAL CHEMISTRY.
Sub Category
ENDOCRINE.
Synonyms/Aliases
LUTEOTROPIC HORMONE; INTERSTITIAL CELL STIMULATING HORMONE; LUTROPHIN.
Abbreviations
LH; ICSH.
Type
INDIVIDUAL TEST
3 HRS
RESULTS READY/ REPORTED IN 3 HOURS.
Method
IMMUNOASSAY.
Temp
AMBIENT, REFRIGERATED (PREFERRED) OR FROZEN.
Setup
DAILY.
Components Parameters
NOT APPLICABLE.
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
NO PREPARATION REQUIRED.
Sample type
SERUM. HEPARIN PLASMA IS AN ACCEPTABLE ALTERNATE SPECIMEN.
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
AT LEAST 2ML.
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
AGE, GENDER, MENSTRUAL PHASE (FOLLICULAR PHASE VS. MID CYCLE PEAK VS. LUTEAL PHASE), POST MENOPAUSAL REFERENCE INTERVALS (AS APPROPRIATE) ARE PROVIDED ON THE REPORT.
Clinical Utility
IN CONJUNCTION WITH FSH, LH IS USED IN ASSESSMENT OF MALE & FEMALE (IN)FERTILITY, AMENORRHEA, OLIGOMENORRHEA, MENSTRUAL IRREGULARITY, MENOPAUSE, HYPOTHALAMIC & PITUITARY DISORDERS, PRECOCIOUS OR DELAYED PUBERTY. INCREASED LH/ FSH RATIO MAY BE SEEN IN POLYCYSTIC OVARIAN SYNDROME (PCOS).
Test Limitations/ Confounders
IMMUNOASSAY INTERFERENCE CAN GIVE RISE TO SPURIOUS RESULTS. SUCH INTERFERENCE CAN ARISE FROM PRESENCE OF HETEROPHILE ANTIBODIES, ANTI-ANIMAL ANTIBODIES, AUTOANTIBODIES, PARAPROTEINS, BIOTIN ETC. RESULT INTERPRETATION IS INFLUENCED BY TANNER STAGING (CHILDREN), MENSTRUAL CYCLE PHASE (IN WOMEN OF REPRODUCTIVE AGE), MENOPAUSE.