Sub Category



Bone Marrow Biopsy, BM Biopsy.




Profile/ panel (bundled tests).

7 Days

Results ready in 7 days.


Tissue processing, staining and microscopy.


Room temperature.



Components Parameters

N/A. However concurrent CBC/ PBF ordering is required.

Pre-analytic Considerations


Test Ordering Instructions

Fill out test request form fully & legibly. Clinical history/ indications required inclusive of results of other diagnostic work-up. Concurrent CBC printout or CBC order required. Concurrent PBF order/ examination required for optimal hematological assessment.

Patient preparation


Sample type

Properly Prepared, Labeled, Air Dried, Fixed Smears Required. Slides Should Be Labeled With 2 Patient Identifiers, Including Name. If Slides Are Not Fixed, Label as Unfixed. If Fixation is done, This Should Be With Freshly Prepared Methanol.

Container/ preservative

Trephine Biopsy should be placed in 10% neutral Buffered Formalin. Additionally if a bone marrow aspirate (BMA) sample is concurrently obtained, BMA slides should be prepared directly. Alternatively, BMA sample can be transferred into EDTA (purple top) Vacutainer but slides must then be prepared within 1-2 hours of collection.

Sample volume

Aim for Trephine Biopsy length ≥15mm.

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Mismatch Between Request Form & Sample Details; Missing Age/ Gender; Wrong Sample Type; Insufficient Sample; Broken Slides

Other Instructions

If additional tests are required, obtain the relevant samples & list them on the request form e.g. Flow cytometry (EDTA & heparin Vacutainer, 3ml each), Cytogenetics (Sodium Heparin Vacutainer), Culture (Bood Culture Bottles) etc.

Reference intervals

Negative for disease / Malignancy.

Clinical Utility

Variable E.g. Assess Unexplained Cytopenias (Anemia, Leukopenia, Thrombocytopenia), Unexplained Cytoses (Polycythemia, Leukocytosis, Thrombocytosis), Suspected Malignancy or Infiltration (Leukemia, Lymphoma, Myeloproliferative Neoplasms, Metastatic Disease), Pyrexia of Unknown Origin, Infectious Diseases (E.g. Leishmaniasis), B Symptoms (Unintentional Weight Loss, Drenching Night Sweats, Fever) Etc.

Test Limitations/ Confounders

Poor BMA Slide Preparation & Fixation Can Make BMA Unreportable; Poor Trephine Biopsy Fixation & Decalcification Can Make Biopsies Unreportable.