Category
SEROLOGY/ IMMUNOLOGY
Sub Category
INFECTIOUS.
Synonyms/Aliases
N/A.
Abbreviations
TPHA
Type
INDIVIDUAL TEST.
3 HRS
RESULTS READY IN 3 HRS.
Method
PARTICLE AGGLUTINATION.
Temp
AMBIENT (24 HOURS), REFRIGERATED (3 DAYS), FROZEN (14 DAYS).
Setup
ALL WORKING DAYS.
Components Parameters
N/A.
PRE-ANALYTIC CONSIDERATIONS
Test Ordering Instructions
FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.
Patient preparation
N/A.
Sample type
SERUM.
Container/ preservative
SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.
Sample volume
2 ML.
Rejection criteria
LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; HEMOLYZED, LIPEMIC, ICTERIC SAMPLES.
Other Instructions
CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.
Reference intervals
NEGATIVE.
Clinical Utility
DIAGNOSIS OF SYPHILIS INFECTION.
Test Limitations/ Confounders
ONCE POSITIVE, REMAINS POSITIVE FOR LIFE IRRESPECTIVE OF TREATMENT (CANNOT DISTINGUISH BETWEEN PAST & CURRENT INFECTION OR TO MONITOR TREATMENT RESPONSE). REQUIRES FOLLOW-UP TESTING WITH A NON-TREPONEMAL TEST E.G. VDRL/ RPR IF NOT ALREADY PERFORMED (NO SINGLE SEROLOGICAL TEST CAN DIAGNOSE ACTIVE SYPHILIS).