Category

Coagulation

SubCategory

Bleeding/ bleeding risk assessment

Abbreviations

VWF AG

Synonyms/Aliases

VWF antigen, factor viii cofactor antigen

Test

Individual Test

Results

results ready aftER 7-10 working Days

Method

Clotting

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly.

Test Considerations

fill out test request form fully & legibly. fasting sample, sample filled up to the mark

Patient Preparations

Fasting , preferably patient should not be on anticoagulants 2 weeks prior to the test

Sample Types

citrated platelet poor plasma (frozen)

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Sample Volume

During sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Diagnose von willebrand factor deficiency

Test Limitations /Confounders

VWF levels can be elevated in many conditions

Other Instructions

To prepare Platelet poor Plasma, Double centrifuge sample immediately after collection i.e. centrifuge, transfer pPasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.