Haematology

Collect whole blood in CITRATE TUBE (LIGHT BLUE CAP) FILLED UP TO THE MARK & submit to the lab immediately at ROOM temperature. If >4 hour delay is expected between collection & analysis, centrifuge, separate plasma & freeze. Submit frozen plasma. Provide history e.g. whether patient is on anticoagulants (e.g. heparin) and whether they have a bleeding tendency. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 2-8 hours at 20-25°C │ Refrigerated: 2-8 hours at 2-8°C │ Frozen: 2 WEEKS at -20°C

EDTA (purple top) vacutainer. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 3 days at 20-25°C │ Refrigerated: 7 days at 2-8°C │ Frozen: 6 moths at -20°C

FROZEN, DOUBLE CENTRIFUGED 2mL CITRATED PLASMA required. Collect whole blood in citrate tube(s) i.e. light blue cap vacutainer filled up to the mark, centrifuge, separate plasma, centrifuge plasma, separate & FREEZE supernatant. For instructions/ further guidance on how to prepare platelet poor plasma, please visit this YouTube video: https://www.youtube.com/watch?v=9z6c8Bgr2EY SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Room Temperature: N/A │ Frozen: 3 months at -20°C

Serum required. It is performed as part of a "type and screen" whenever a blood transfusion is anticipated, as part of prenatal testing of pregnant women or in the investigation of transfusion reactions. Confirm indication prior to testing. Recheck any positive ICT result occurring in a woman who is not Rhesus D negative & has never been transfused.

FROZEN, DOUBLE CENTRIFUGED 2mL CITRATED PLASMA required. Collect whole blood in citrate tube(s) i.e. light blue cap vacutainer filled up to the mark, centrifuge, separate plasma, centrifuge plasma, separate & FREEZE supernatant. For instructions/ further guidance on how to prepare platelet poor plasma, please visit this YouTube video: https://www.youtube.com/watch?v=9z6c8Bgr2EY SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Room Temperature: N/A │ Refrigerated: 4 days at 2-8°C │ Frozen: 1 month at -20°C

3ml EDTA (purple top vacutainer) and/ or sodium heparin (green top vacutainer) whole blood. Components include: % and absolute counts of CD3 (total T lymphocytes), CD4 (helper T cells), CD8 (suppressor T cells), CD19 (total B lymphocytes).

During sample collection, fill up to the mark on the tube (do not ""add up"" samples collected in other tubes to reach the required volume). Submit 2mL frozen citrated plasma in a separation vial. The aliquot label should identify the sample type as citrated plasma. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 8 HOURS at 20-25°C │ Refrigerated: 4 DAYS at 2-8°C │ Frozen: 6 MONTHS at -20°C

FROZEN, DOUBLE CENTRIFUGED 2mL CITRATED PLASMA required. Collect whole blood in citrate tube(s) i.e. light blue cap vacutainer filled up to the mark, centrifuge, separate plasma, centrifuge plasma, separate & FRFEEZE supernatant. For instructions/ further guidance on how to prepare platelet poor plasma, please visit this YouTube video: https://www.youtube.com/watch?v=9z6c8Bgr2EY SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Room Temperature: N/A │ N/A │ Frozen: 1 month at -20°C

Heparin (green top) vacutainer and smear freshly prepared from EDTA vacutainer. Test components: Whole blood, NaCl solutions of varying concentrations, Red blood cells, Hemolysis observation

1) Liaise with lab team for booking of direct clients
2) Name & contact details of the referring doctor must be available
3) Previous patient records & test results, imaging inclusive to be availed
4) Recent CBC, <=72 hours old needed (concurrent CBC can be obtained at time of the procedure; PBF examination will still be required unless recently performed at Scanlab).
5) Collect a concurrent serum sample since other tests are frequently required.
6) For <12-14 year olds, anaesthesia is required at additional cost (for young children, may have to be done as an inpatient procedure)
Includes sample collections for PBF and BM aspirate WITHOUT trephine biopsy or clot section. For guidance on preparation of peripheral blood films, review this YouTube video: https://www.youtube.com/watch?v=kafqQZmrgQE For guidance on preparation of bone marrow aspirate films, review this YouTube video: https://www.youtube.com/watch?v=t_yOm3jaCCI For guidance on packaging of slides for transportation, review this YouTube video: https://www.youtube.com/watch?v=u-Vy80b_-sI&t=2s