POLARIZING MICROSCOPY, CRYSTALS.

Category

Microbiology

Sub Category

Fluid Analysis

Synonyms/Aliases

Uric Acid Crystals; Crystals, Synovial/ Joint Fluid

Abbreviations

N/A -

Type

Individual Tests

Results

Results will be ready within 5 days

Method

Microscopy (Polarized Light)

Temp

Transport temprature kept Refrigirated

Setup

Setup perfomed everyday

Pre-analytic Considerations :

.

Components / Parameters

N / A

Test ordering Instructions

Fill out test request form fully & legibly.

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Synovial Fluid Sample

Container/ preservative

EDTA (Purple Top) Or Heparin (Green Top). Samples In Red Top (Serum; Plain) Vacutainer Or In Plastic Separation Vials May Be Accepted If Not Clotted.

Sample volume

3 ML (minimum 1ml) synovial fluid.

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Frozen Sample; Clotted Sample; SST (GEL) Tube.

Other Instructions

N / A -

Reference intervals

Negative (no crystals seen)

Clinical Utility

To Evaluate For Pathologic Crystals & Diagnose Gout And Pseudogout (Calcium Pyrophosphate Dihydrate Crystal Deposition Disease, CPPD).

Test Limitations/ Confounders

N / A

MYCOBACTERIUM TUBERCULOSIS CULTURE

Category

Microbiology

Sub Category

Mycobacteriology (TB; MOTT)

Synonyms/Aliases

TB Culture

Abbreviations

N/A

Type

Profile/ Panel (BUNDLED TESTS)

Results

Results will be ready within 7 weeks

Method

Culture and antibiotic sensitivity testing (where indicated) using conventional identification methods & KIRBY BAUER method for antibiotic susceptibility testing.

Temp

Transport temprature kept at 2-8ºC

Setup

Setup perfomed everyday

Pre-analytic Considerations :

.

Components / Parameters

Pathogen isolation, identification & reporting of appropriate Antibiotics as applicable

Test ordering Instructions

Fill out test request form fully & legibly. Specify exact sample type/ anatomic site.

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Variable (SPUTUM, FLUID, TISSUE, BLOOD etc.)

Container/ preservative

Sterile Container

Sample volume

3 ML for Fluids

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details

Other Instructions

N / A

Reference intervals

No Growth Obtained/ No Pathogens Isolated.

Clinical Utility

Detection of M. Tuberculosis.

Test Limitations/ Confounders

N / A

MICROSCOPY, CULTURE & SENSITIVITY (AEROBIC)

Category

Microbiology

Sub Category

Microscopy/ Culture/ Sensitivity (M/C/S)

Synonyms/Aliases

Culture & sensitivity

Abbreviations

M/C/S

Type

Profile/ Panel (BUNDLED TESTS)

Results

3-5 days depending on sample type (or as soon as positive results are obtained)

Method

Culture and antibiotic sensitivity testing (where indicated) using conventional identification methods & KIRBY BAUER method for antibiotic susceptibility testing.

Temp

Room temperature (swabs)

Setup

Setup perfomed everyday

Pre-analytic Considerations :

.

Components / Parameters

Pathogen isolation, identification & reporting of appropriate Antibiotics as applicable

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Sample site & specific sample type must be specified.

Container/ preservative

Sterile Container

Sample volume

If a swab is collected, submit two in transport media; if fluid is collected, submit 3-5ml.

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

For specific instructions for blood, stool, urine, HVS, CSF culture, refer to specific test for appropriate instructions.

Reference intervals

No Growth Obtained/ No Pathogens Isolated.

Clinical Utility

Evaluation of suspected infection.

Test Limitations/ Confounders

Prior exposure to antibiotics can result in false negatiive cultures.

INDIA INK STAIN

Category

Microbiology

Sub Category

Microscopy

Synonyms/Aliases

India Ink

Abbreviations

N / A

Type

Individual Test

Results

Results reported within the same day

Method

Microscopic examination after staining

Temp

Room temperature, 2-8ºc (if culture is to be done on the same sample, please do not refrigerate; transport to lab immediately)

Setup

The setup is Perfomed All working days

Pre-analytic Considerations :

.

Components / Parameters

N / A

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample type

C S F

Container/ preservative

Sterile Container

Sample volume

3 Milliliters

Rejection criteria

Labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample label details; incorrect sample type.

Other Instructions

N / A

Reference intervals

Negative

Clinical Utility

Diagnosis of Cryptococcus Neoformans infection.

Test Limitations/ Confounders

Lower sensitivity than cryptococcal antigen (CRAG) test.

H PYLORI ANTIGEN (STOOL)

Category

Microbiology

Sub Category

Antigen Test

Synonyms/Aliases

Helicobacter Pylori Antigen

Abbreviations

H. pylori Ag

Type

Individual Test

Result

Results are ready after 2 hours

Method

Immunochromatography

Temp

Transportation Temperature needs to be Refrigirated

Setup

The setup is Perfomed Daily

Pre-analytic Considerations

N/A

Components / Parameters

N / A

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample type

Stool

Container/ preservative

Clean , Leak proof container

Sample volume

10 gRAMS

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

N / A

Reference intervals

Negative

Clinical Utility

Diagnosis Of Helicobacter Pylori Infection & evaluation of treatment success.

Test Limitations/ Confounders

N / A

FUNGAL STAIN (KOH)

Category

Microbiology

Sub Category

Microscopy

Synonyms/Aliases

Fungal Stain

Abbreviations

K O H

Type

Individual Test

Result

Results are ready in 24 hours

Method

Stain / Microscopy

Temp

Transportation at Room temperature

Setup

The setup is Perfomed Daily

Pre-analytic Considerations

N/A

Components / Parameters

N / A

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample type

Variable including nail clippings, skin scrapings, hair

Container/ preservative

Sterile Container

Sample volume

N / A

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

If culture is required, this must be specifically ordered. Cut hair is not ideal for testing; the root of the hair is required. Skin scrapings can be obtained using the blunt edge of a scapel; target the active (outermost) border of the lesion.

Reference intervals

Negative / No Fungal Elements seen

Clinical Utility

To Diagnose Fungal Infections e.g. Dermatophytosis.

Test Limitations/ Confounders

N / A

FUNGAL MICROSCOPY, CULTURE & IDENTIFICATION

Category

Microbiology

Sub Category

Microscopy/ culture/ sensitivity (M/C/S)

Synonyms/Aliases

Fungal microscopy, culture & identification

Abbreviations

Fungal M/C/S

Type

Profile/ panel (bundled tests)

Results

Results are ready in 21-28 days (or as soon as positive results are obtained)

Method

Culture & pathogen identification using conventional methods. If indicated, antifungal susceptibility testing is ordered at additional cost.

Temp

Transportation at Room temperature (swabs)

Setup

The setup is Perfomed Daily

Pre-analytic Considerations

N/A

Components / Parameters

Pathogen isolation, identification & reporting of appropriate antibiotics as applicable

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample type

STOOL

Container/ preservative

Clean, leak proof container

Sample volume

If a swab is collected, submit two in transport media; if fluid is collected, submit 3-5ml.

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

For specific instructions for blood, stool, urine, hvs, csf culture, refer to specific test for appropriate instructions.

Reference intervals

No growth obtained/ no pathogens isolated

Clinical Utility

Evaluation of suspected mycoses including recovery and identification of dermatophyte fungi.

Test Limitations/ Confounders

N / A

FECAL FAT, QUALITATIVE

Category

Microbiology

Sub Category

Stool Examination

Synonyms/Aliases

Fecal Fat

Abbreviations

N / A

Type

Individual Test

Results

Results reported after 5 days

Method

N / A

Temp

Transportation at Room temperature (slides)

Setup

The setup is Perfomed All working days

Pre-analytic Considerations

N/A

Components / Parameters

N / A

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample type

STOOL

Container/ preservative

Clean, leak proof container

Sample volume

50 Miligrams

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

N / A

Reference intervals

Negative

Clinical Utility

Determines the presence or absence of excess fat

Test Limitations/ Confounders

N / A

CEREBROSPINAL FLUID (CSF) CULTURE & SENSITIVITY (AEROBIC)

Category

Microbiology

Sub Category

Microscopy / Culture /Sensitivity (M/C/S)

Synonyms/Aliases

Spinal fluid culture and sensitivity

Abbreviations

CSF C/S; CSF M/C/S

Type

Profile/ panel (bundled tests)

5 days

Results ready in 5 days (or as soon as positive results are obtained)

Method

Culture and antibiotic sensitivity testing (where indicated) using conventional methods for identification & kirby bauer method for antibiotic susceptibility testing.

Temp

Room temperature (slides)

Setup

All working days

Pre-analytic Considerations

N/A

Components / Parameters

Pathogen isolation, identification & reporting of appropriate antibiotics as applicable

Test ordering Instructions

If being transported, csf must be stored at room temperature at room temperature and delivered immediately to the laboratory. Does not include cell counts (order separately)

Patient preparation

N / A

Sample type

C S F

Container/ preservative

Sterile Container

Sample volume

At least 0.5-1ML of csf in sterile leak proof container. If multiple tests are required, minimum is 5ml divided between 3-4 sequentially labelled tubes.

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

CSF should ideally be collected in multiple tubes labeled in accordance with the collection sequence. When this is done, tube 2 is used for microbiological tests.

Reference intervals

No Growth Obtained/ No Pathogens Isolated.

Clinical Utility

CNS infections E.G. Meningitis.

Test Limitations/ Confounders

Prior antibiotic exposure, sample refrigeration may result in false negative cultures.

CRYPTOSPORIDIUM

Category

Microbiology

Sub Category

Microscopy

Synonyms/Aliases

Blood Culture

Abbreviations

N/A

Type

Individual Test

5 days

5 Days. Positive Results Are Reported As Soon As They Are Obtained. Negative Results Are Reported At 48 Hours (Interim) And 5 Days (Final)

Method

Staining & Microscopy

Temp

Room temperature (slides)

Setup

All working days

Pre-analytic Considerations

N/A

Components / Parameters

N / A

Test ordering Instructions

Fill out test request form fully & legibly

Patient preparation

N / A

Sample Requirements

Fill out test request form fully & legibly. clinical history/ indications required inclusive of results of other diagnostic work-up. concurrent CBC printout or CBC order required. concurrent PBF order/ examination required for optimal hematological assessment.

Sample type

Stool

Container/ preservative

Sterile Container

Sample volume

10 Grams

Rejection criteria

Labeling Issue (Unlabeled/ Mislabeled); Missing Request Form; Incorrect Preservative/ Container; Frozen Sample.

Other Instructions

N / A

Reference intervals

No Growth Obtained/ No Pathogens Isolated.

Clinical Utility

Establishing the diagnosis of intestinal cryptosporidiosis.

Test Limitations/ Confounders

N / A