Routine chemistry – ScanLab Center

12 Dec

TOTAL PROTEIN, SERUM

December 12, 2022June 8, 2023vincentochiengde

Category

ROUTINE CHEMISTRY

Sub Category

ORGAN FUNCTION CHECKS

Synonyms/Aliases

N/A

Abbreviations

^Type

INDIVIDUAL TEST

^{7 Days}

RESULTS READY/ REPORTED IN 3 HOURS

^Method

SPECTROPHOTOMETRIC

^Temp

ROOM TEMPERATURE 7 DAYS; 2-8ºC 7 DAYS; FROZEN 30 DAYS

^Setup

DAILY

Components Parameters

NOT APPLICABLE.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM. HEPARIN PLASMA IS AN ACCEPTABLE ALTERNATIVE.

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

AT LEAST 3ML.

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.

Other Instructions

SERUM GEL TUBES SHOULD BE CENTRIFUGED WITHIN 1-2 HOURS OF COLLECTION. RED-TOP TUBES SHOULD BE CENTRIFUGED AND ALIQUOTED WITHIN 1-2 HOURS OF COLLECTION. MINIMIZE TOURNIQUET TIME DURING COLLECTION.

Reference intervals

AGE AND GENDER APPROPRIATE VALUES ARE PROVIDED WITH THE REPORT.

Clinical Utility

USED IN SCREENING & DIAGNOSIS OF LIVER DISEASE, PROTEIN LOSING NEPHROPATHY, PROTEIN LOSING ENTEROPATHY. LEVELS MAY BE INCREASED IN MULTIPLE MYELOMA, CHRONIC ACTIVE INFLAMMATORY STATES ETC.

Test Limitations/ Confounders

- N/A

12 Dec

SERUM PROTEIN ELECTROPHORESIS (SPE)

December 12, 2022June 7, 2023vincentochiengde

Category

SPECIAL CHEMISTRY.

Sub Category

TUMOR MARKER.

Synonyms/Aliases

PROTEIN ELECTROPHORESIS, SERUM.

Abbreviations

SPE.

^Type

PROFILE/ PANEL (BUNDLED TESTS).

^{5 Days}

RESULTS READY/ REPORTED IN 5 WORKING DAYS.

^Method

SCANNING DENSITOMETRY OR CAPILLARY ELECTROPHORESIS.

^Temp

REFRIGERATED.

^Setup

BATCHED.

Components Parameters

TOTAL PROTEIN AND FRACTIONS (ALBUMIN, ALPHA 1, ALPHA 2, BETA 1, BETA 2, GAMMA GLOBULIN FRACTIONS).

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM.

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

AT LEAST 2ML.

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.

Other Instructions

CENTRIFUGE SST (SERUM GEL TUBES) WITHIN 1-2 HOURS OF COLLECTION. CENTRIFUGE RED TOP VACUTAINERS AND ALIQUOT SERUM WITHIN 1-2 HOURS OF COLLECTION. THE SEPARATION VIAL MUST BE LABELED WITH AT LEAST 2 PATIENT IDENTIFIERS AND THE SAMPLE TYPE.

Reference intervals

NEGATIVE FOR AN M-BAND/ M-SPIKE/ PARAPROTEIN.

Clinical Utility

DIAGNOSE & MONITOR PATIENTS WITH MONOCLONAL GAMMOPATHIES E.G. MULTIPLE MYELOMA.

Test Limitations/ Confounders

PSEUDOPARAPROTEINEMIA CAN OCCUR E.G. DUE TO TESTING PLASMA (FIBRINOGEN BAND) INSTEAD OF SERUM, CONTRAST INTERFERENCE ETC. LOW OR NEGATIVE MONOCLONAL BAND MAY OCCUR IN LIGHT CHAIN MYELOMA BUT THE M-BAND WILL BE DETECTED IN URINE.

12 Dec

CALCIUM, TOTAL

December 12, 2022June 7, 2023vincentochiengde

Category

Routine Chemistry

Sub Category

Electrolytes

Synonyms/Aliases

N/A

Abbreviations

CA; CA2+

^Type

INDIVIDUAL TEST

^Results

RESULTS READY IN 2-3WORKING DAYS

^Method

SPECTROPHOTOMETRIC

^Temp

AMBIENT: 6HOURS; R (2-8℃): 7DAYS; F (-20℃): 14DAYS

^Setup

ALL WORKING DAYS

Components Parameters

- N/A

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

NOT APPLICABLE

Sample type

SERUM

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER

Sample volume

AT LEAST 2 ML

Rejection criteria

HEMOLYZED SAMPLE; LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; GROSS LIPEMIA; GROSS HEMOLYSIS.

Other Instructions

NOT APPLICABLE

Reference intervals

APPROPRIATE VALUES ARE PROVIDED ON THE REPORT.

Clinical Utility

DIAGNOSIS AND MONITORING OF A WIDE RANGE OF DISORDERS INCLUDING DISEASES OF BONE, KIDNEY, PARATHYROID GLAND OR GASTROINTESTINAL TRACT. TO RULE IN/ OUT HYPOCALCEMIA & HYPERCALCEMIA.

Test Limitations/ Confounders

HYPOALBUMINEMIA CAN AFFECT TOTAL CALCIUM LEVELS AND ALBUMIN-ADJUSTED CALCIUM LEVELS ARE REQUIRED IN THE PRESENCE OF HYPOALBUMINEMIA

12 Dec

75g ORAL GLUCOSE TOLERANCE TEST

December 12, 2022June 7, 2023vincentochiengde

Category

Routine chemistry

Sub Category

Endocrine

Synonyms/Aliases

N/A

Abbreviations

OGTT; 75g OGTT

^Type

profile/ panel (bundled tests)

^{3-5 days}

RESULTS READY AFTER 4 HOURS

^Method

SPECTROPHOTOMETRIC ENZYMATIC

^Temp

TEMPRATURE BE REFRIGIRATED

^Setup

ALL WORKING DAYS

Components Parameters

0 hours (fasting), 2-3 hours post load.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

FASTING FOR AT LEAST 8 HOURS REQUIRED.

Sample type

FLUORIDE/ OXALATE PLASMA. MULTI-SAMPLING IS REQUIRED I.E. BASELINE FASTING SAMPLE; THEN 1 & 2 HOUR POST LOAD SAMPLES. SAMPLE LABELS MUST INCLUDE COLLECTION SEQUENCE E.G. 0 (BASELINE), 1HR, 2HR SAMPLE

Container/ preservative

FLUORIDE/ OXALATE (GREY TOP) VACUTAINER

Sample volume

AT LEAST 1 ML FLUORIDE/ OXALATE PLASMA EACH.

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; GROSS HEMOLYSIS; NONFASTING SAMPLES.

Other Instructions

URINE SAMPLES FOR URINE GLUCOSE TESTING ARE NOT ROUTINELY COLLECTED.

Reference intervals

AS PER THE LATEST AMERICAN DIABETIC ASSOCIATION CONSENSUS GUIDELINES.

Clinical Utility

SCREENING & DIAGNOSIS OF PREDIABETES & DIABETES MELLITUS (DM)

Test Limitations/ Confounders

NOT INTENDED FOR PREGNANT WOMEN/ DIAGNOSIS OF GDM. FOR THISN, REFER TO 75g OGTT, GESTATIONAL

12 Dec

UREA

December 12, 2022June 8, 2023vincentochiengde

Category

ROUTINE CHEMISTRY

Sub Category

ORGAN FUNCTION CHECKS

Synonyms/Aliases

BLOOD UREA NITROGEN

Abbreviations

BUN

^Type

INDIVIDUAL TEST

^Results

results ready in 3 HOURS

^Method

SPECTROPHOTOMETRIC

^Temp

ROOM TEMPERATURE 7 DAYS; REFRIGERATED, 2-8ºC, 7 DAYS; FROZEN 30 DAYS

^Setup

DAILY

Components Parameters

N/A; however concurrent CBC/ PBF ordering is required.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

NOT APPLICABLE

Sample type

SERUM (HEPARIN PLASMA IS AN ACCEPTABLE ALTERNATIVE).

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

AT LEAST 2 ML.

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; GROSS LIPEMIA.

Other Instructions

SERUM GEL TUBES SHOULD BE CENTRIFUGED WITHIN 1-2 HOURS OF COLLECTION. RED-TOP TUBES SHOULD BE CENTRIFUGED AND ALIQUOTED WITHIN 1-2 HOURS OF COLLECTION.

Reference intervals

AGE AND GENDER APPROPRIATE VALUES ARE PROVIDED WITH REPORT

Clinical Utility

USEFUL IN THE EVALUATION OF KIDNEY FUNCTION AND IN MONITORING PATIENTS UNDERGOING DIALYSIS.

Test Limitations/ Confounders

- -N/A

12 Dec

CREATINE KINASE MB FRACTION

December 12, 2022June 7, 2023vincentochiengde

Category

Routine Chemistry

Sub Category

Cardiovascular Risk Assessment

Synonyms/Aliases

Creatine Phosphokinase-mb; Ck Isoenzyme Mb

Abbreviations

CK-MB; CPK-MB

^Type

INDIVIDUAL TEST

^Results

RESULTS READY IN 2 - 3 HOURS

^Method

SPECTROPHOTOMETRY

^Temp

AMBIENT: 8HOURS; R (2-8℃): 7DAYS; F (-20℃): 30DAYS

^Setup

ALL WORKING DAYS

Components Parameters

NOT APPLICABLE

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

NOT APPLICABLE

Sample type

SERUM

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER

Sample volume

AT LEAST 2 ML

Rejection criteria

Other Instructions

NOT APPLICABLE

Reference intervals

AGE & GENDER APPROPRIATE VALUES ARE PROVIDED ON THE REPORT.

Clinical Utility

TO DIAGNOSE OR RULE OUT HEART DISEASE E.G. HEART ATTACK (ACUTE MYOCARDIAL INFARCTION). HOWEVER TROPONIN IS A SUPERIOR BIOMARKER.

Test Limitations/ Confounders

PRESENCE OF MACRO CK CAN LEAD TO SPURIOUSLY INCREASED CK-MB LEVELS.

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12 Dec

GAMMA GLUTAMYL TRANSFERASE

December 12, 2022June 8, 2023vincentochiengde

Category

Routine Chemistry

Sub Category

Organ Function Checks

Synonyms/Aliases

Gamma Gt

Abbreviations

GGT

^Type

INDIVIDUAL TEST

^Results

RESULTS READY AFTER 2-3 HOURS

^Method

QUANTITATIVE ENZYMATIC SPECTROPHOTOMETRIC

^Temp

AMBIENT: 6HOURS; R (2-8℃): 7DAYS; F (-20℃): 14DAYS

^Setup

DAILY

Components Parameters

NOT APPLICABLE

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

NOT APPLICABLE

Sample type

SERUM

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER

Sample volume

2 ML

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE; GROSS HEMOLYSIS OR LIPEMIA.

Other Instructions

NOT APPLICABLE

Reference intervals

AGE & GENDER APPROPRIATE VALUES ARE PROVIDED ON THE REPORT.

Clinical Utility

DIAGNOSE & MONITOR HEPATOBILIARY DISEASE.

Test Limitations/ Confounders

PRESENCE OF MACRO GGT CAN LEAD TO SPURIOUSLY INCREASED GGT LEVELS.

12 Dec

LIPID PROFILE

December 12, 2022June 8, 2023vincentochiengde

Category

ROUTINE CHEMISTRY

Sub Category

CARDIOVASCULAR RISK ASSESSMENT

Synonyms/Aliases

LIPOGRAM; FASTING LIPID PROFILE.

Abbreviations

- N/A

^Type

PROFILE/ PANEL (BUNDLED TESTS)

^Results

RESULTS READY AFTER 2-3 HOURS

^Method

HOMOGENEOUS ENZYMATIC SPECTROPHOTOMETRIC TEST

^Temp

AMBIENT: 1DAY; R (2-8℃): 7DAYS; F (-20℃): 14D

^Setup

SETUP IS PERFORMED DAILY

Components Parameters

TOTAL CHOLESTEROL, LDL CHOLESTEROL, HDL CHOLESTEROL, NON-HDL CHOLESTEROL, TRIGLYCERIDES.

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY. FASTING IS NOT ROUTINELY REQUIRED. FOR SELECT PATIENTS, FASTING LIPID PROFILE MAY BE MORE APPROPRIATE. WHERE STIPULATED BY THE ORDERING DOCTOR, 9-12 HOURS FASTING IS REQUIRED

Patient preparation

FASTING IS NOT ROUTINELY REQUIRED. WHERE STIPULATED BY THE ORDERING DOCTOR, 9-12 HOURS FASTING IS REQUIRED. FASTING MEANS THAT ONLY WATER AND PRESCRIBED MEDICATION ARE PERMITTED DURING THE FASTING PERIOD.

Sample type

SERUM

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER

Sample volume

AT LEAST 2 ML

Rejection criteria

Other Instructions

NOT APPLICABLE

Reference intervals

CONSENSUS MEDICAL DECISION POINTS ARE PROVIDED ONN THE REPORT FOR INTERPRETATION.

Clinical Utility

DIAGNOSIS OF CARDIOVASCULAR RISK, DYSLIPIDEMIA, HYPOLIPOPROTEINEMIAS.

Test Limitations/ Confounders

- N/A

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12 Dec

TRANSFERRIN

December 12, 2022June 8, 2023vincentochiengde

Category

ROUTINE CHEMISTRY

Sub Category

ANEMIA & NUTRITION

Synonyms/Aliases

N/A

Abbreviations

N/A

^Type

INDIVIDUAL TEST

^Results

RESULTS READY IN 3 DAYS

^Method

NEPHELOMETRY

^Temp

ROOM TEMPERATURE 2-3 HOURS, 2-8ºC 7 DAYS, FROZEN 30 DAYS

^Setup

DAILY

Components Parameters

N/A

PRE-ANALYTIC CONSIDERATIONS

Test Ordering Instructions

FILL OUT TEST REQUEST FORM FULLY & LEGIBLY.

Patient preparation

N/A

Sample type

SERUM

Container/ preservative

SERUM SEPARATOR TUBE (SST/ GEL) OR PLAIN (RED TOP) VACUTAINER.

Sample volume

3 ML

Rejection criteria

LABELING ISSUE (UNLABELED/ MISLABELED); MISSING REQUEST FORM; MISMATCH BETWEEN REQUEST FORM & SAMPLE DETAILS; MISSING AGE/ GENDER; WRONG SAMPLE TYPE; INSUFFICIENT SAMPLE.

Other Instructions

SERUM GEL TUBES SHOULD BE CENTRIFUGED WITHIN 1-2 HOURS OF COLLECTION. RED-TOP TUBES SHOULD BE CENTRIFUGED AND ALIQUOTED WITHIN 1-2 HOURS OF COLLECTION.

Reference intervals

AGE AND GENDER APPROPRIATE VALUES ARE PROVIDED WITH THE REPORT.

Clinical Utility

ASSESS IRON BALANCE AND A DIRECT MEASURE OF THE IRON BINDING CAPACITY

Test Limitations/ Confounders

- N/A