Special chemistry

Serum in plain tube (red top) or SST tube (yellow top) or in a separation vial. Centrifuge tubes within 1 hour. Separate centrifuged serum in plain tubes within 1 hour. When the order simply states vitamin D, select 25-hydroxyvitamin D (C1014) instead of 1,25 dihydroxyvitamin D (C1015). SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 3 DAYS at 20-25°C │ Refrigerated: 7 DAYS at 2-8°C │ Frozen: 3 MONTHS at -20°C

Overnight fasting; PPIs to be discontinued 7 days prior to sampling (with consent of attending doctor). SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 7 days at 20-25°C │ Refrigerated: 7 days at 2-8°C │ Frozen: 1 month at -20°C

PATIENT PREPARATION/ POSTURE: Patient should be upright/ ambulatory for at least 2 hours prior to sampling. If patient is not ambulatory and a supine collection was done, this MUST be clearly stated on the request form BLOOD COLLECTION: 3-5 mL EDTA plasma. TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Frozen: 3 months at -20°C HANDLING (EDTA PLASMA): Keep chilled e.g. keep samples on ice slurry immediately after collection. Centrifuge within 30 minutes to separate plasma. Transfer EDTA plasma to a plastic vial without disturbing blood cells. Label properly with 2 patient identifiers and sample type i.e. EDTA plasma. FREEZE. TRANSPORT AND STORAGE (EDTA PLASMA): Freezing: Freeze EDTA plasma immediately at -20°C or lower. Record relevant patient information on the TRF i.e. posture (MANDATORY i.e. supine vs upright), medications.

Overnight fasting; separate & FREEZE serum sample.

24 hour urine collection is required; add 6M HCl as preservative to the 24 hour urine container; explain collection instructions [refer to relevant phlebotomy aid (Phleb/Aid7) or lab handbook section on 24 hour urine collection or https://www.youtube.com/watch?v=HjF-9n0FOrw video]. Submit 10mL aliquot of 24 hour urine. TOTAL 24 hour urine volume (in mL) is required (mandatory).

EDTA plasma 2mL frozen SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Frozen ANALYTE STABILITY Room Temperature: 1 day at 20-25°C │ Refrigerated: 2 days at 2-8°C │ Frozen: 1 month at -20°C

1-2mL serum. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 3 DAYS at 20-25°C │ Refrigerated: 3 DAYS at 2-8°C │ Frozen: 6 MONTHS at -20°C