16 May

VON WILLEBRAND FACTOR ANTIGEN

vincentochiengde

Category

Coagulation

SubCategory

Bleeding/ bleeding risk assessment

Abbreviations

VWF AG

Synonyms/Aliases

VWF antigen, factor viii cofactor antigen

Test

Individual Test

Results

results ready aftER 7-10 working Days

Method

Clotting

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly.

Test Considerations

fill out test request form fully & legibly. fasting sample, sample filled up to the mark

Patient Preparations

Fasting , preferably patient should not be on anticoagulants 2 weeks prior to the test

Sample Types

citrated platelet poor plasma (frozen)

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Sample Volume

During sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Diagnose von willebrand factor deficiency

Test Limitations /Confounders

VWF levels can be elevated in many conditions

Other Instructions

To prepare Platelet poor Plasma, Double centrifuge sample immediately after collection i.e. centrifuge, transfer pPasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
15 May

ANTITHROMBIN III, ACTIVITY

vincentochiengde

Category

Coagulation

SubCategory

Thrombosis/ Thrombosis risk assessment

Abbreviations

AT III, AT3

Synonyms/Aliases

Antithrombin activity, AT3 activity, Heparin co-factor activity

Test

Individual Test

Results

results ready aftER 7-10 working Days

Method

Chromogenic activity assay

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

fasting preferred to avoid lipemia. patient should not be on anticoagulants 2 weeks prior to the test

Sample Types

citrated platelet poor plasma (frozen)

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Examine / monitor Antithrombin Deficiency

Test Limitations /Confounders

Anticoagulant treatment may falsely decrease the levels of protein C; activation of coagulation pathway can deplete protein c levels; this is a temperature sensitive analyte; frozen handling required.

Other Instructions

To prepare Platelet poor Plasma, Double centrifuge sample immediately after collection i.e. centrifuge, transfer pPasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
15 May

PROTEIN S, ACTIVITY

vincentochiengde

Category

Coagulation

SubCategory

Thrombosis/ Thrombosis risk assessment

Abbreviations

N/A

Synonyms/Aliases

Protein S functional, Functional Protein S,

Test

Individual Test

Results

results ready aftre 7-10 working Days

Method

Chromogenic activity assay

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly. filled up to the mark, fasting sample

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

patient should not be on Anticoagulants 2 weeks prior to the test, fasting

Sample Types

citrated platelet poor plasma (frozen)

Sample Volume

During sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Diagnosing Protein c Deficiency

Test Limitations /Confounders

Anticoagulant treatment may falsely decrease the levels of protein C; activation of coagulation pathway can deplete protein c levels; this is a temperature sensitive analyte; frozen handling required.

Other Instructions

To prepare Platelet poor Plasma, Double centrifuge sample immediately after collection i.e. centrifuge, transfer pPasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
15 May

PROTEIN C , ACTIVITY

vincentochiengde

Category

Coagulation

SubCategory

Thrombosis/ Thrombosis risk assessment

Abbreviations

PRO-C,

Synonyms/Aliases

functional protein c, protein c functional

Test

Individual Test

Results

results ready aftre 7-10 working Days

Method

Chromogenic activity assay

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly. filled up to the mark, fasting sample

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

patient should not be on anticoagulants 2 weeks prior to the test,

Sample Types

citrated platelet poor plasma (frozen)

Sample Volume

During sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Diagnosing Protein c Deficiency

Test Limitations /Confounders

Anticoagulant treatment may falsely decrease the levels of protein c; activation of coagulation pathway can deplete protein c levels; this is a temperature sensitive analyte; frozen handling required.

Other Instructions

To prepare platelet poor plasma, double centrifuge sample immediately after collection i.e. centrifuge, transfer plasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
15 May

LUPUS ANTICOAGULANT (LA) PANEL

vincentochiengde

Category

Coagulation

SubCategory

Thrombosis/ Thrombosis risk assessment

Abbreviations

LAC, dRVVT, RVVT

Synonyms/Aliases

Lupus Anticoagulant profile, APTT mixing studies, dilute russel viper venom time (DRVVT)

Test

profile/ panel (bundled tests)

Results

results ready aftre 7-10 working Days

Method

Mixed E.g. Time To Clotting (APTT); Mixing Studies, Calculation Of Ratios

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

APTT, Dilute Russel Viper Venom Time (DRVVT), Mixing Studies

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly.

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

fasting preferred to avoid lipemia.

Sample Types

citrated platelet poor plasma (frozen)

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 4ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Rule out LUPUS anticoagulant; evaluation of thrombotic states.

Test Limitations /Confounders

Anticoagulants may result to false positive results. this is a temperature sensitive analyte; frozen handling required.

Other Instructions

To prepare platelet poor plasma, double centrifuge sample immediately after collection i.e. centrifuge, transfer plasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
15 May

FACTOR VIII, ACTIVITY

vincentochiengde

Category

Coagulation

SubCategory

bleeding/ bleeding risk assessment

Abbreviations

AHF

Synonyms/Aliases

Factor 8, Factor Viii Assay, Antihemophilic Factor, Hemophilia A, Anti-hemophilia A

Test

Individual Test

Results

results ready aftre 7-10 working Days

Method

chromogenic activity assay

Temp

Transportation temperature need to be Frozen

Setup

Setup is Conducted Once a week

Pre-analytic Considerations

.

Component Parameters

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly.

Container / Presavative

Citrate (light blue top) vacutainer during sample collection.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

fasting preferred to avoid lipemia.

Sample Types

citrated platelet poor plasma (frozen)

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; wrong sample type; insufficient sample; hemolyzed samples; underfilled tubes, clotted samples, lipemic samples.

Reference intervals

Appropriate values are provided on the report

Clinical utility

Examination of prolonged APTT, diagnosis of VWF deficiency, diagnosis of Hemophilia A

Test Limitations /Confounders

this is a temperature sensitive analyte; frozen handling required.

Other Instructions

To prepare platelet poor plasma, double centrifuge sample immediately after collection i.e. centrifuge, transfer plasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
15 May

FACTOR VII, ACTIVITY

vincentochiengde

Category

Coagulation

SubCategory

bleeding/ bleeding risk assessment

Abbreviations

N/A

Synonyms/Aliases

proconvertin, factor vii assay

Test

Individual Test

Results

results ready aftre 7-10 working Days

Method

chromogenic activity assay

Temp

Transportation temperature need to be Frozen

Setup

Once a week

Component Parameters

N/A

PRE-ANALYTIC CONSIDERATIONS

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly.

Container / Presavative

Citrate (light blue top) vacutainer during sample collection.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

fasting preferred to avoid lipemia.

Sample Types

citrated platelet poor plasma (frozen)

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml platelet poor (double centrifuged) frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; wrong sample type; insufficient sample; hemolyzed samples; underfilled tubes, clotted samples, lipemic samples.

Reference intervals

appropriate values are provided on the report

Clinical utility

evaluate factor vii deficiency, evaluate prolonged INR

Test Limitations /Confounders

Refrigerating sample can result to conversion of factor vii to activated factor viia

Other Instructions

to prepare platelet poor plasma, double centrifuge sample immediately after collection i.e. centrifuge, transfer plasma, centrifuge plasma a second time & transfer the supernatant which should now be platelet poor. freeze immediately.
12 Dec

PROTHROMBIN TIME / INTERNATIONAL NORMALISED RATIO

vincentochiengde

Category

Coagulation

SubCategory

bleeding/ bleeding risk assessment

Abbreviations

PT/INR

Synonyms/Aliases

prothrombin time assay,

Test

profile/ panel (bundled tests)

Results

results ready 2-4 hours after receiving sample.

Method

Time to clot formation

Temp

room temperature (24 hours maximum stability), frozen (30 days)

Setup

All working Days

Pre-analytic Considerations

.

Component Parameters

INR, Test Time, ISI

Sample Requirements

Citrated whole blood/ plasma

Test Ordering Instructions

fill out test request form fully & legibly. fill out test request form fully & legibly. provide clinical history including whether the patient is on anticoagulants e.g. warfarin or has liver disease.

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

N/A

Sample Types

citrated whole blood / plasma

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). if submitting separated citrated plasma, the aliquot label should identify the sample type as citrated plasma & 1-2ml submitted.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; wrong sample type; insufficient sample; hemolyzed samples; underfilled tubes, clotted samples, lipemic samples.

Other Instructions

sample should be separated after collection for transport

Reference intervals

test and control values are provided with report

Clinical utility

monitor patients on unfractionated heparin (ufh) therapy. examine factor deficiency in the extrinsic & common coagulation cascade pathway

Test Limitations /Confounders

Underfilled tubes, incorrect handling will give rise to wrong results. Patients with high hematocrit >55% require specially prepared tubes for correct results to be obtained.
12 Dec

ACTIVATED PARTIAL THROMBOPLASTIN TIME (aPTT)

vincentochiengde

Category

Coagulation

Sub Category

bleeding/ bleeding risk assessment

Synonyms/Aliases

partial Thromboplastin time, Kaolin Cephalin clotting time

Abbreviations

APTT, PTT, KCCT

Test

Indivudual Test

Results

2-4 hours after receiving sample.

Temp

room temperature (4 hours stability), frozen plasma (30 days)

Method

time to clot formation

Setup

All working days

Component / Parameters

.N / A

Pre-analytic Considerations

N / A

Sample Requirements

Citrated whole blood/ plasma

Patient Preparations

Citrated whole blood/ plasma

Sample Type

Citrated whole blood/ plasma

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). if submitting separated citrated plasma, the aliquot label should identify the sample type as citrated plasma & 1-2ml submitted.

Test Ordering Instructions

fill out test request form fully & legibly. provide clinical history including whether the patient is on anticoagulants e.g. unfractionated heparin (ufh) or has liver disease.

Container Presavative

Citrate(LIGHT BLUE TOP) Vacutainer

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; wrong sample type; insufficient sample; hemolyzed samples; underfilled tubes, clotted samples, lipemic samples; frozen whole blood samples.

Other Instructions

Send citrated whole blood at room temperature; alternatively send separated plasma at room temperature (must be tested within 4 hours of collection) or frozen plasma. Should not be haemolyzed or clotted. Samples ?4 hours old received at room temperature are rejected; special tubes should be prepared for clients with hematocrit ?55% sample should be separated after collection for transport

Reference intervals

test and control values are provided with report

Clinical Utility

monitor patients on unfractionated heparin (UFH) therapy. examine factor deficiency in the intrinsic & common coagulation cascade pathway

Test Limitations /Confounders

monitor patients on unfractionated heparin (UFH) therapy. examine factor deficiency in the intrinsic & common coagulation cascade pathway
12 Dec

D-DIMER

vincentochiengde

Category

Coagulation

SubCategory

bleeding & Thrombosis assessment

Abbreviations

N / A

Synonyms/Aliases

N/A

Test

Individual Test

Results

results ready after 3 hours

Method

Turbidimetric Or Immunoassay

Temp

Transported temprature be Frozen(PLASMA)

Setup

done all working days

Pre-analytic Considerations

.

Component Parameters

N/A

Test Orderin Instructions

N/A

Sample Requirements

Citrated whole blood/ plasma

Test Considerations

fill out test request form fully & legibly.

Patient Preparations

N/A

Sample Types

citrated platelet poor plasma (frozen)

Sample Volume

during sample collection, fill up to the mark on the tube (do not "add up" samples collected in other tubes to reach the required volume). submit 2ml frozen citrated plasma in a separation vial. the aliquot label should identify the sample type as citrated plasma.

Container / Presavative

citrate (light blue top) vacutainer during sample collection.

Rejection Criteria

labeling issue (unlabeled/ mislabeled); missing request form; mismatch between request form & sample details; missing age/ gender; wrong sample type; insufficient sample; hemolyzed samples; clotted samples; incorrect temperature handling.

Other Instructions

samples at room temperature should be received in 4hours

Reference intervals

appropriate values provided with report

Clinical utility

diagnose disseminated intravascular coagulation, monitoring response to therapeutic fibrinolysis

Test Limitations /Confounders

d-dimer levels can be increased in various clinical disorders e.g. recent trauma, surgery, inflammation, neoplasia etc.
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