MOLECULAR PATHOLOGY

3ml EDTA, whole blood, and clinical history.

3mL urine sample (commonest specimen type) Other alternate sample types: Urethral swab, endocervical swab, first morning/ first void (first portion) urine sample. If a urine sample is submitted, patient should not have urinated for at least 1 hour prior to collection Test components: CT, NG, MG, TV.

5 ml of serum or EDTA Plasma. Freeze immediately after separation. NB: Order MUST be clarified if the doctor simply states HBV PCR i.e. clarification on whether qualitative PCR should be done (used for DIAGNOSIS; code MP2313) or whether quantitative PCR/ viral load should be done (used for TREATMENT MONITORING where diagnosis is ALREADY established; code MP2310). Test components: HEPATITIS B VIRAL LOAD, SAMPLE TYPE SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Refrigerated ANALYTE STABILITY Room Temperature: 7 days at 20-25°C │ Refrigerated: 1 month at 2-8°C │ Frozen: 1 month at -20°C

Sample type: EDTA whole blood or bone marrow. Container/ preservative: EDTA (purple top) vacutainer Sample volume: 3mL Shipping Temperature: Refrigerated Test components: P210 (E13A2, E14A2) major transcript, BCR::ABL1/ABL1 % (IS), sample type (peripheral blood vs bone marrow).

Peripheral blood in sodium heparin vacutainer. Clinical history/ indication required. Specific form to be filled before sending out.

Specimen must be collected at 10 weeks gestation or greater. Testing will be canceled for specimens collected at less than 10 weeks of gestation. Number of fetuses must be provided (this info is available on the ultrasound report). Testing will be canceled if number of fetuses is not provided. Copy of ultrasound report must be provided. Patient's date of birth, gestational age on the date of collection, pregnancy type (singleton or multiple), and the clinical indications (including advanced maternal age, abnormal ultrasound, history suggestive of increased risk for aneuploidy, positive serum screen, or other indications) must be captured on the test request form. Draw 10 mL whole blood in special Streck/ blood cfDNA tube. Ambient temperature. Test components: 1. Nuclear cfDNA 2. Mitochondrial cfDNA 3. Tumor-derived cfDNA (ctDNA) 4. Fetal cfDNA 5. Microbial cfDNA

3ml of EDTA Whole Blood in EDTA

3mL EDTA (purple top) whole blood. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Ambient ANALYTE STABILITY Room Temperature: 1 days at 20-25°C │ Refrigerated: 7 days at 2-8°C │ Frozen: 6 weeks at -20°C

3mL EDTA plasma. SHIPPING/ TRANSPORTATION REQUIREMENTS Shipping Temperature: Ambient ANALYTE STABILITY Room Temperature: 1 days at 20-25°C │ Refrigerated: 7 days at 2-8°C │ Frozen: 6 weeks at -20°C

SAMPLE TYPE: Tumor positive formalin-fixed, paraffin-embedded (FFPE) block with adequate residual tissue. CONTAINER/ PRESERVATIVE: Formalin fixed, paraffin embedded blocks [preferred]. Alternatively, 5 microns unstained sections on positively-charged slides (≥four slides) & 1 matching H&E slide.